Document Detail

The role of gibberellins in embryo axis development.
MedLine Citation:
PMID:  12147724     Owner:  NLM     Status:  MEDLINE    
The role of gibberellins (GAs) during early embryo development was examined using microspore-derived embryos (MDEs) of Brassica napus. At the globular stage of development, 10 d after initial culture (DAC) when endogenous GA(1) levels are increasing rapidly, a triazole, uniconazole, was used at 1, 33 and 100 microM to inhibit GA biosynthesis. Within this dose range there was no apparent effect of the inhibitor on embryo growth through to the early torpedo stage. However, by 25 DAC uniconazole-treated MDEs showed significantly reduced (50%) axis elongation. Addition of GA(1) at 33 microM on 14 DAC to embryos pretreated with 1 microM uniconazole on 10 DAC prevented this reduction in axis length, giving axis elongation equivalent to untreated MDEs. Application of GA(1) alone, however, did not significantly increase axis elongation. The reduced axis growth seen with uniconazole treatment was due to reduced cell elongation, but not cell number, and the co-applied GA(1) thus prevented the uniconazole-induced reduction in cell length. The elongating axis of MDEs may thus be a useful tool for examining the role of GAs in cell elongation.
Dirk B Hays; Edward C Yeung; Richard P Pharis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of experimental botany     Volume:  53     ISSN:  0022-0957     ISO Abbreviation:  J. Exp. Bot.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-30     Completed Date:  2002-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9882906     Medline TA:  J Exp Bot     Country:  England    
Other Details:
Languages:  eng     Pagination:  1747-51     Citation Subset:  IM    
Plant Physiology Research Group, Department of Biological Sciences, 2500 University Drive NW, University of Calgary, Calgary, Alberta, Canada T2N IN4.
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MeSH Terms
Brassica napus / drug effects,  growth & development
Dose-Response Relationship, Drug
Gibberellins / antagonists & inhibitors,  metabolism*,  pharmacology
Seeds / drug effects,  growth & development*,  metabolism
Time Factors
Triazoles / pharmacology
Reg. No./Substance:
0/Gibberellins; 0/Triazoles; 83657-22-1/uniconazole

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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