Document Detail


The role of extracellular matrix, integrins, and cytoskeleton in mechanotransduction of centrifugal loading.
MedLine Citation:
PMID:  18026855     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The study was aimed to investigate the role of the "extracellular matrix (ECM)-integrins-cytoskeleton" signal pathway in mechanotransduction of centrifugal loading. MG-63 osteoblasts were exposed to centrifugal loading at 209xg for 10 min. Uncentrifuged cells and centrifuged cells that have been trypsinized and suspended in liquors were designed as control. The changes in F-actin and alpha-actin cytoskeleton, gene transcription of ECM components, and integrins expression were analyzed by LSCM, Real-Time RT-PCR and FCM, respectively. A temporary and fast reversible change was observed in F-actin and alpha-actin cytoskeleton. And the change was paralleled with the fast autoregulation in gene transcription of ECM components of fibronection, osteopontin and Collagen I, and integrins expression of both alpha2 and beta1 subunits. The result suggested that cytoskeleton was a possible mechanical sensor to centrifugal stimuli, and the cytoskeleton regulation to centrifugal loading was in an ECM-dependent and integrin-mediated manner.
Authors:
Juan Li; Zhihe Zhao; Jun Wang; Guoping Chen; Jingyuan Yang; Songjiao Luo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-16
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  309     ISSN:  0300-8177     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-24     Completed Date:  2008-05-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  41-8     Citation Subset:  IM    
Affiliation:
Department of Orthodontics, West China College of Stomatology, Sichuan University, 14#, 3rd section, Renmin South Road, Chengdu 610041, PR China.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism
Antigens, CD29 / metabolism*
Cell Line
Centrifugation
Collagen Type I / genetics,  metabolism
Cytoskeleton / metabolism*
Extracellular Matrix / metabolism*
Fibronectins / genetics,  metabolism
Gene Expression Regulation
Humans
Integrin alpha2 / metabolism*
Mechanotransduction, Cellular*
Osteopontin / genetics,  metabolism
RNA, Messenger / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Actins; 0/Antigens, CD29; 0/Collagen Type I; 0/Fibronectins; 0/Integrin alpha2; 0/RNA, Messenger; 106441-73-0/Osteopontin

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