| The role of Na+/Ca2+ exchanger in endothelin-1-aggravated hypoxia/reoxygenation-induced injury in renal epithelial cells. | |
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MedLine Citation:
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PMID: 17446489 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We analyzed the role of the Na+/Ca2+ exchanger (NCX) in endothelin-1-aggravated hypoxia/reoxygenation-induced injury in renal epithelial LLC-PK1 cells. KB-R7943, a selective NCX inhibitor, suppressed hypoxia/reoxygenation-induced cell damage, whereas overexpression of NCX1 into cells enhanced it. Endothelin-1 significantly aggravated hypoxia/reoxygenation-induced injury in parental and NCX1-overexpressing LLC-PK1 cells. Such aggravation by endothelin-1 was not observed in cells overexpressing a deregulated NCX1 mutant, which displays no protein kinase C-dependent activation. These results suggest that Ca2+ overload via NCX plays a critical role in hypoxia/reoxygenation-induced renal tubular injury, and that endothelin-1 aggravates the cell damage through the activation of NCX. |
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Authors:
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Satomi Kita; Ayako Furuta; Yukio Takano; Takahiro Iwamoto |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Annals of the New York Academy of Sciences Volume: 1099 ISSN: 0077-8923 ISO Abbreviation: Ann. N. Y. Acad. Sci. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-04-20 Completed Date: 2007-05-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7506858 Medline TA: Ann N Y Acad Sci Country: United States |
Other Details:
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Languages: eng Pagination: 473-7 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anoxia / physiopathology* Endothelin-1 / physiology* Epithelial Cells / pathology Kidney / pathology* LLC-PK1 Cells Oxygen / metabolism* Swine Thiourea / analogs & derivatives, pharmacology |
| Chemical | |
Reg. No./Substance:
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0/2-(2-(4-(4-nitrobenzyloxy)phenyl)ethyl)isothiourea methanesulfonate; 0/Endothelin-1; 62-56-6/Thiourea; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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