Document Detail


The role of Na+/Ca2+ exchanger in endothelin-1-aggravated hypoxia/reoxygenation-induced injury in renal epithelial cells.
MedLine Citation:
PMID:  17446489     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We analyzed the role of the Na+/Ca2+ exchanger (NCX) in endothelin-1-aggravated hypoxia/reoxygenation-induced injury in renal epithelial LLC-PK1 cells. KB-R7943, a selective NCX inhibitor, suppressed hypoxia/reoxygenation-induced cell damage, whereas overexpression of NCX1 into cells enhanced it. Endothelin-1 significantly aggravated hypoxia/reoxygenation-induced injury in parental and NCX1-overexpressing LLC-PK1 cells. Such aggravation by endothelin-1 was not observed in cells overexpressing a deregulated NCX1 mutant, which displays no protein kinase C-dependent activation. These results suggest that Ca2+ overload via NCX plays a critical role in hypoxia/reoxygenation-induced renal tubular injury, and that endothelin-1 aggravates the cell damage through the activation of NCX.
Authors:
Satomi Kita; Ayako Furuta; Yukio Takano; Takahiro Iwamoto
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1099     ISSN:  0077-8923     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-04-20     Completed Date:  2007-05-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  473-7     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, School of Medicine, Fukuoka University, Fukuoka 814-0180, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Anoxia / physiopathology*
Endothelin-1 / physiology*
Epithelial Cells / pathology
Kidney / pathology*
LLC-PK1 Cells
Oxygen / metabolism*
Swine
Thiourea / analogs & derivatives,  pharmacology
Chemical
Reg. No./Substance:
0/2-(2-(4-(4-nitrobenzyloxy)phenyl)ethyl)isothiourea methanesulfonate; 0/Endothelin-1; 62-56-6/Thiourea; 7782-44-7/Oxygen

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