| The role of estrogen receptor subtypes on hepatic neutrophil accumulation following trauma-hemorrhage: direct modulation of CINC-1 production by Kupffer cells. | |
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MedLine Citation:
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PMID: 18468914 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Although 17beta-estradiol (E2) administration following trauma-hemorrhage (T-H) reduces liver injury by decreasing neutrophil accumulation via estrogen receptor (ER)-alpha, it remains unclear whether cytokine-induced neutrophil chemoattractant (CINC)-1 production by Kupffer cells (KC) is directly modulated by ER-alpha under such condition. Male rats underwent laparotomy and hemorrhagic shock (40 mmHg for 90 min), followed by resuscitation with four times the shed blood volume in the form of Ringer's lactate. ER-alpha agonist propyl pyrazole triol (PPT; 5 microg/kg), ER-beta agonist diarylpropionitrile (DPN; 5 microg/kg), E2 (50 microg/kg), or vehicle (10% DMSO) was administered subcutaneously during resuscitation; rats were sacrificed 24h thereafter. KC were isolated and cultured with ER agonists to examine if they directly affect CINC-1 production. T-H increased plasma alanine aminotransferase (ALT; hepatic injury) and hepatic myeloperoxidase (MPO) activity. E2, PPT and DPN administration reduced increased ALT; however, PPT was more effective than DPN. PPT and E2, but not DPN significantly attenuated increased hepatic MPO activity and CINC-1 levels. PPT addition in vitro (10(-7) and 10(-6)M) significantly reduced KC CINC-1 production. In summary, the salutary effects of E2 against hepatic injury are mediated predominantly via ER-alpha which directly modulates KC CINC-1 production and hepatic neutrophil accumulation following T-H. |
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Authors:
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Tomoharu Shimizu; Takao Suzuki; Huang-Ping Yu; Yukihiro Yokoyama; Mashkoor A Choudhry; Kirby I Bland; Irshad H Chaudry |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-05-12 |
Journal Detail:
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Title: Cytokine Volume: 43 ISSN: 1096-0023 ISO Abbreviation: Cytokine Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-24 Completed Date: 2008-08-12 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 9005353 Medline TA: Cytokine Country: United States |
Other Details:
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Languages: eng Pagination: 88-92 Citation Subset: IM |
Affiliation:
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Center for Surgical Research and Department of Surgery, University of Alabama at Birmingham, G094 Volker Hall, 1670 University Boulevard, Birmingham, AL 35294-0019, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Movement / drug effects, physiology Chemokine CXCL1 / biosynthesis*, blood Disease Models, Animal Estrogen Receptor alpha / agonists, physiology* Estrogen Receptor beta / agonists, physiology* Female Hemorrhage / metabolism, pathology* Kupffer Cells / metabolism*, physiology Liver Diseases / metabolism*, pathology*, therapy Male Neutrophil Infiltration / drug effects, physiology* Nitriles / administration & dosage Propionates / administration & dosage Pyrazoles / administration & dosage Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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R37 GM039519-21/GM/NIGMS NIH HHS; R37 GM39519/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chemokine CXCL1; 0/Cxcl1 protein, rat; 0/Estrogen Receptor alpha; 0/Estrogen Receptor beta; 0/Nitriles; 0/Propionates; 0/Pyrazoles; 0/diarylpropionitrile; 0/propyl pyrazole triol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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