Document Detail


The role of enzymology in a structure-based drug discovery program: bacterial DNA gyrase.
MedLine Citation:
PMID:  22222453     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The capability to accurately, rapidly, and reproducibly determine the affinity of a ligand for a target protein or enzyme is a vital component for a successful structure-based drug design effort. In order to successfully drive a structure-based drug design (SBDD) project forward, multiple distinct assays, each with particular strengths and weaknesses, need to be employed. Using bacterial DNA gyrase as an example, a range of assays are described that will fully support an SBDD program.
Authors:
Mark L Cunningham
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  841     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-01-06     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  179-207     Citation Subset:  IM    
Affiliation:
Trius Therapeutics, San Diego, CA, USA, mcunningham@triusrx.com.
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