Document Detail

The role of electrolytes and pH in RBC ASs.
MedLine Citation:
PMID:  11493737     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Experimental additive solutions (EASs) containing saline, adenine, glucose, mannitol and disodium phosphate can support RBCs for 9 or 10 weeks if used in 200- or 300-mL volumes. The effects of variations in the electrolyte composition and volume of EASs were explored. STUDY DESIGN AND METHODS: In three four-arm studies, 24 RBC units were pooled in groups of 4 and realiquoted as test units to ensure that all donors were equally represented in each study arm. In Study 1, units were stored for 11 weeks in EAS containing 0, 10, 20, or 30 mmol per L of sodium bicarbonate. In Study 2, units were stored for 9 weeks in EAS containing 26, 50, 100, or 150 mmol per L of sodium chloride. In Study 3, units were stored in 100 or 200 mL of AS-3 or EAS-61. RBC ATP concentrations and hemolysis were measured weekly. RESULTS: Increasing the sodium bicarbonate content of EASs increased the pH throughout storage and increased RBC ATP concentrations in the later phases of storage, but it had no effect on hemolysis. Increased sodium chloride content of EASs led to lower RBC ATP concentrations and increased hemolysis. In EAS-61, RBC ATP concentrations were increased throughout storage, and hemolysis was lower than that of RBCs stored in AS-3. CONCLUSION: RBC ATP synthesis is highly dependent on the pH of the AS. Hemolysis is affected by the salt content and volume of the AS.
J R Hess; N Rugg; A D Knapp; J F Gormas; H R Hill; C K Oliver; L E Lippert; T J Greenwalt
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Transfusion     Volume:  41     ISSN:  0041-1132     ISO Abbreviation:  Transfusion     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-08-08     Completed Date:  2001-08-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417360     Medline TA:  Transfusion     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1045-51     Citation Subset:  IM    
Blood Research Detachment, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA.
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MeSH Terms
Adenosine Triphosphate / analysis
Blood Preservation / methods*,  standards
Dose-Response Relationship, Drug
Electrolytes / pharmacology
Erythrocytes / drug effects*,  metabolism
Hydrogen-Ion Concentration
Pharmaceutical Solutions / chemistry,  pharmacology*
Sodium Bicarbonate / pharmacology
Sodium Chloride / pharmacology
Time Factors
Reg. No./Substance:
0/Electrolytes; 0/Pharmaceutical Solutions; 144-55-8/Sodium Bicarbonate; 56-65-5/Adenosine Triphosphate; 7647-14-5/Sodium Chloride

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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