Document Detail

The role of cyanide in the removal of type 2 copper from laccase.
MedLine Citation:
PMID:  7625834     Owner:  NLM     Status:  MEDLINE    
We have improved a method for the removal of the type 2 copper from tree laccase under anaerobic reducing conditions and developed a mechanistic model. We identify two key steps in the reaction: (i) dissociation of copper(I) catalyzed by trace levels of cyanide in a weakly acidic medium and (ii) sequestration of the released metal by an appropriate chelator such as 2,9-dimethyl-1,10-phenanthroline. We maintain a steady-state concentration of cyanide in a pH 5.5 acetate buffer under a constantly exchanging nitrogen atmosphere by introducing a cyanometalate ion as a cofactor or by continuously injecting the ion into the protein solution. The type 2-depleted product is identical to previous preparations as regards its spectral properties, activity level and ability to recombine with copper(I). The mechanistic insights appear to be quite general and should form the basis for the development of methods for removing the type 2 copper from other related systems.
M K Eggleston; C Pecoraro; D R McMillin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  320     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1995 Jul 
Date Detail:
Created Date:  1995-08-28     Completed Date:  1995-08-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  276-9     Citation Subset:  IM    
Department of Chemistry, Purdue University, West Lafayette, Indiana 47907-1393, USA.
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MeSH Terms
Copper / chemistry*,  metabolism
Cyanides / metabolism*
Oxidoreductases / chemistry*,  metabolism
Plants / enzymology
Reg. No./Substance:
0/Cyanides; 7440-50-8/Copper; EC 1.-/Oxidoreductases; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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