Document Detail

Continuous hypoxia attenuates paraquat-induced cytotoxicity in the human A549 lung carcinoma cell line.
MedLine Citation:
PMID:  21734449     Owner:  NLM     Status:  MEDLINE    
Paraquat (1,1'-dimethyl-4,4'-bipyridinium dichloride; PQ), an effective and widely used herbicide, was commercially introduced in 1962. It is reduced by the electron donor NADPH, and then reduced PQ transfers the electrons to molecular oxygen, resulting in the production of reactive oxygen species (ROS), which are related to cellular toxicity. However, the influence of continuous hypoxia on PQ-induced ROS production has not fully been investigated. We evaluated in vitro the protective effect of continuous hypoxia on PQ-induced cytotoxicity in the human carcinogenic alveolar basal epithelial cell line (A549 cells) by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and live and dead assay, and by measuring lactate dehydrogenase (LDH) release. To elucidate the mechanism underlying this effect, we monitored the immunofluorescence of intracellular ROS and measured malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities. Continuous hypoxia protected the A549 cells from PQ-induced cytotoxicity. Continuous hypoxia for a period of 24 h significantly reduced intracellular ROS, decreased MDA concentration in the supernatant, and normalized SOD and GPx activities. Continuous hypoxia attenuated PQ-induced cell toxicity in A549 cells. This protective effect might be attributable to the suppression of PQ-induced ROS generation.
Hoon Kim; Suk Woo Lee; Kyung Min Baek; Jung Soo Park; Jin Hong Min
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental & molecular medicine     Volume:  43     ISSN:  2092-6413     ISO Abbreviation:  Exp. Mol. Med.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-30     Completed Date:  2012-02-03     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  9607880     Medline TA:  Exp Mol Med     Country:  Korea (South)    
Other Details:
Languages:  eng     Pagination:  494-500     Citation Subset:  IM    
Department of Emergency Medicine, Chungbuk National University College of Medicine, Cheongju 361-763, Korea.
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MeSH Terms
Cell Hypoxia
Cell Line, Tumor
Glutathione Peroxidase / metabolism
Herbicides / toxicity*
L-Lactate Dehydrogenase / metabolism
Lung / cytology*,  drug effects*,  metabolism
Malondialdehyde / metabolism
Oxidative Stress
Paraquat / toxicity*
Reactive Oxygen Species / metabolism*
Superoxide Dismutase / metabolism
Reg. No./Substance:
0/Herbicides; 0/Reactive Oxygen Species; 4685-14-7/Paraquat; 542-78-9/Malondialdehyde; EC Dehydrogenase; EC Peroxidase; EC Dismutase

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