Document Detail


A role for the chemokine receptor CCR6 in mammalian sperm motility and chemotaxis.
MedLine Citation:
PMID:  23765988     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Although recent evidence indicates that several chemokines and defensins, well-known as inflammatory mediators, are expressed in the male and female reproductive tracts, the location and functional significance of chemokine networks in sperm physiology and sperm reproductive tract interactions are poorly understood. To address this deficiency in our knowledge, we examined the expression and function in sperm of CCR6, a receptor common to several chemoattractant peptides, and screened several reproductive tract fluids for the presence of specific ligands. CCR6 protein is present in mouse and human sperm and mainly localized in the sperm tail with other minor patterns in sperm from mice (neck and acrosomal region) and men (neck and midpiece regions). As expected from the protein immunoblotting and immunofluorescence results, mouse Ccr6 mRNA is expressed in the testis. Furthermore, the Defb29 mRNA encoding the CCR6 ligand, β-defensin DEFB29, is expressed at high levels in the epididymis. As determined by protein chip analysis, several chemokines (including some that act through CCR6, such as CCL20/MIP-3α (formerly macrophage inflammatory protein 3α) and protein hormones were present in human follicular fluid, endometrial secretions, and seminal plasma. In functional chemotaxis assays, capacitated human sperm exhibited a directional movement towards CCL20, and displayed modifications in motility parameters. Our data indicate that chemokine ligand/receptor interactions in the male and female genital tracts promote sperm motility and chemotaxis under non-inflammatory conditions. Therefore, some of the physiological reactions mediated by CCR6 ligands in male reproduction extend beyond a pro-inflammatory response and might find application in clinical reproduction and/or contraception.
Authors:
Pedro Caballero-Campo; Mariano G Buffone; Fabian Benencia; José R Conejo-García; Paolo F Rinaudo; George L Gerton
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  229     ISSN:  1097-4652     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  2014 Jan 
Date Detail:
Created Date:  2013-09-30     Completed Date:  2013-12-09     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  68-78     Citation Subset:  IM    
Copyright Information:
© 2013 Wiley Periodicals, Inc.
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MeSH Terms
Descriptor/Qualifier:
Animals
Chemokine CCL20 / biosynthesis,  genetics
Chemotaxis / genetics*
Epididymis / cytology,  metabolism
Female
Gene Expression Regulation, Developmental
Humans
Male
Mice
Receptors, CCR6 / biosynthesis*,  genetics
Sperm Motility / genetics*
Spermatozoa / growth & development,  metabolism*,  ultrastructure
beta-Defensins / biosynthesis,  genetics
Grant Support
ID/Acronym/Agency:
2R01CA124515/CA/NCI NIH HHS; CA137499-01/CA/NCI NIH HHS; HD-41552/HD/NICHD NIH HHS; HD-RO1 062803-01A1/HD/NICHD NIH HHS; R01 CA124515/CA/NCI NIH HHS; R01 CA157664/CA/NCI NIH HHS; R01 HD041552/HD/NICHD NIH HHS; R01 HD062803/HD/NICHD NIH HHS; R01 TW008662/TW/FIC NIH HHS; R01CA157664/CA/NCI NIH HHS; R01TW008662/TW/FIC NIH HHS; R15 CA137499/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/CCL20 protein, human; 0/CCR6 protein, human; 0/Chemokine CCL20; 0/Receptors, CCR6; 0/beta-Defensins
Comments/Corrections

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