Document Detail

The role of cells versus matrix in bone induction.
MedLine Citation:
PMID:  2186889     Owner:  NLM     Status:  MEDLINE    
Earlier observations indicated that epithelial cells of urinary bladder, and transformed epithelial cells from human amnion (FL), epidermal carcinoma (HeLa), etc. can induce ectopic endochondral bone formation when implanted into the skeletal muscle of immunosuppressed or autologous animals. Such epithelial cells are associated with little matrix. Bone-inducing activity was also demonstrated in cultured osteosarcoma cells of murine and human origin or extracts thereof, and it is notable that these bone-inducing osteosarcoma cells grow in vitro with little matrix production. Finally, electron microscopy of in vitro cartilage induction showed that decalcified rodent bone that had been extensively extracted to remove cells still contained devitalized cells and cell fragments some of which made contact with inducible, cartilage-forming mesenchymal cells that had migrated in from cocultured muscle. Suggestions: These observations suggest that: 1) the bone-inducing agent(s) of both epithelial and mesenchymal cells may reside mostly in cells rather than matrix. Thus it may be premature to assume that bone "matrix" is the major source of bone-inducing agent in decalcified bone until the osteoinductive activity of residual bone cells has been assessed; and 2) that the osteoinductive agent, whether residing in epithelial cells or bone cells, may be the same or a similar factor operating through the same mechanism.
H C Anderson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Review    
Journal Detail:
Title:  Connective tissue research     Volume:  24     ISSN:  0300-8207     ISO Abbreviation:  Connect. Tissue Res.     Publication Date:  1990  
Date Detail:
Created Date:  1990-06-21     Completed Date:  1990-06-21     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0365263     Medline TA:  Connect Tissue Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3-12     Citation Subset:  IM; S    
Department of Pathology, University of Kansas Medical Center, Kansas City 66103.
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MeSH Terms
Bone Development / physiology*
Bone Matrix / physiology*
Bone and Bones / physiology
Cartilage / growth & development
Epithelial Cells
Epithelium / physiology
Mesoderm / cytology,  physiology*
Osteosarcoma / physiopathology
Tumor Cells, Cultured
Grant Support

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