Document Detail


The role of cell differentiation state and HMG-I/Y in the expression of transgenes flanked by matrix attachment regions.
MedLine Citation:
PMID:  11708656     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The tobacco nuclear matrix attachment region (MAR), RB7, has been shown to have a much greater effect on transgene expression in cultured cells than in transgenic plants. This is comparable to work in mouse systems showing that MARs have a positive effect on transgene expression in embryonic tissues but not adult tissues. There are several possible explanations for these observations. One is that cell differentiation state and proliferation rate can affect MAR function. We tested this possibility by initiating suspension cell cultures from well-characterized transgenic plants transformed with 35S::GUS with and without flanking MARs and then comparing GUS specific activity in the cell lines to those of the transgenic plants from which the cell lines were derived. If cell differentiation state and proliferation rate do affect MAR function, we would expect the ratio of transgene expression (cell suspensions : plants) to be greater in MAR lines than in control lines. This turned out not to be the case. Thus, it appears that MAR function is not enhanced simply because cells in culture divide rapidly and are not differentiated. Because in animal systems the chromosomal protein HMG-I/Y has been shown to be upregulated in proliferating cells and may have a role in MAR function, we have also examined the levels of the tobacco HMG-I/Y homolog by immunoblotting. The level of this protein does not differ between primary transformant cultured cells (NT-1) and Nicotiana tabacum plants (SR-1). However, a higher molecular weight cross-reacting polypeptide was found in nuclei from the NT-1 cell suspensions but was not detected in SR-1 leaf nuclei or cell suspensions derived from the SR-1 plants.
Authors:
R Ascenzi; J L Ingram; M Massel; W F Thompson; S Spiker; A K Weissinger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Transgenic research     Volume:  10     ISSN:  0962-8819     ISO Abbreviation:  Transgenic Res.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-11-15     Completed Date:  2002-04-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9209120     Medline TA:  Transgenic Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  465-70     Citation Subset:  IM    
Affiliation:
Department of Botany, North Carolina State University, Raleigh 27695-7612, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation
Cells, Cultured
Gene Expression
HMGA1a Protein / genetics*,  metabolism
Plants, Genetically Modified*
Tobacco / cytology,  genetics*
Chemical
Reg. No./Substance:
124544-67-8/HMGA1a Protein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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