| The role of the cell cycle machinery in resumption of postembryonic development. | |
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MedLine Citation:
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PMID: 15579664 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell cycle activity is required for plant growth and development, but its involvement in the early events that initiate seedling development remains to be clarified. We performed experiments aimed at understanding when cell cycle progression is activated during seed germination, and what its contribution is for proper seedling establishment. To this end, the spatial and temporal expression profiles of a large set of cell cycle control genes in germinating seeds of Arabidopsis (Arabidopsis thaliana) and white cabbage (Brassica oleracea) were analyzed. The in vivo behavior of the microtubular cytoskeleton was monitored during Arabidopsis seed germination. Flow cytometry of Arabidopsis germinating seeds indicated that DNA replication was mainly initiated at the onset of root protrusion, when germination reached its end. Expression analysis of cell cycle genes with mRNA in situ localization, beta-glucuronidase assays, and semiquantitative reverse transcription-polymerase chain reaction showed that transcription of most cell cycle genes was detected only after completion of germination. In vivo green fluorescent protein analysis of the microtubule cytoskeleton demonstrated that mitosis-specific microtubule arrays occurred only when the radicle had started to protrude, although the assembly of the microtubular cytoskeleton was promptly activated once germination was initiated. Thus, seed germination involves the synthesis and/or activation of a reduced number of core cell cycle proteins, which only trigger DNA replication, but is not sufficient to drive cells into mitosis. Mitotic divisions are observed only after the radicle has protruded and presumably rely on the de novo production of other cell cycle regulators. |
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Authors:
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Rosa Maria Barrôco; Kris Van Poucke; Jan H W Bergervoet; Lieven De Veylder; Steven P C Groot; Dirk Inzé; Gilbert Engler |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-12-03 |
Journal Detail:
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Title: Plant physiology Volume: 137 ISSN: 0032-0889 ISO Abbreviation: Plant Physiol. Publication Date: 2005 Jan |
Date Detail:
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Created Date: 2005-01-12 Completed Date: 2005-05-24 Revised Date: 2010-09-20 |
Medline Journal Info:
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Nlm Unique ID: 0401224 Medline TA: Plant Physiol Country: United States |
Other Details:
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Languages: eng Pagination: 127-40 Citation Subset: IM |
Affiliation:
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Department of Plant Systems Biology, Flanders Interuniversity Institute for Biotechnology (VIB), Ghent University, B-9052 Gent, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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cytology,
genetics,
growth & development,
metabolism Brassica / cytology, genetics, growth & development, metabolism Cell Cycle / genetics, physiology* Cell Cycle Proteins / genetics Cell Nucleus / metabolism DNA, Plant / metabolism Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Germination / physiology* Plants, Genetically Modified Seeds / cytology, growth & development, physiology* |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/DNA, Plant |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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