| The role of cell cycle-dependent neuropathy target esterase in cell proliferation. | |
| | |
MedLine Citation:
|
PMID: 20306302 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Neuropathy target esterase (NTE) is a novel phospholipase B and plays a role in phospholipid homeostasis. Although over-expression of NTE inhibits cell division, the role of NTE in cell proliferation is still unknown. In the current study, we firstly used synchronous HeLa cells to study the expression profile of NTE during the cell cycle. NTE protein and activity are regulated during the cell cycle with highest level at G1 and lowest at G2/M phase. However, NTE mRNA levels are constant during the cell cycle. The role of NTE in cell proliferation was investigated by short hairpin RNA (shRNA) to suppress the expression of NTE. Knockdown of NTE significant down-regulated of NTE expression and reduced the glycerophosphocholine level. However, suppression of NTE did not affect phosphatidylcholine content or cell cycle progression. In addition, NTE was demonstrated to be degraded by the ubiquitin-proteasome pathway. These results suggested for the first time that NTE is a cell cycle-dependent protein, but is not essential for cell proliferation, and the ubiquitin-mediated proteolysis may be involved in the regulation of NTE during the cell cycle. |
| | |
Authors:
|
Ping-An Chang; Yu-ying Chen; Wen-zhen Qin; Ding-Xin Long; Yi-Jun Wu |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-03-21 |
Journal Detail:
|
Title: Molecular biology reports Volume: 38 ISSN: 1573-4978 ISO Abbreviation: Mol. Biol. Rep. Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2010-12-02 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0403234 Medline TA: Mol Biol Rep Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 123-30 Citation Subset: IM |
Affiliation:
|
Key Laboratory of Molecular Biology, College of Bio-Information, Chongqing University of Posts and Telecommunications, Chongqing, 400065, People's Republic of China. changpingan@yahoo.com.cn |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Three novel SNPs in the coding region of PPAR? gene and their associations with meat quality traits ...
Next Document: A genetic investigation of Korean mummies from the Joseon Dynasty.