Document Detail


The role of caveolin1 and sprouty1 in genistein's regulation of vascular smooth muscle cell and endothelial cell proliferation.
MedLine Citation:
PMID:  20828553     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Genistein prevents atherosclerosis by exerting protective effects on blood vessels. The aim of this study is to investigate the role of caveolin1 and sprouty1 in the regulation of proliferation of vascular smooth muscle cell (VSMC) and endothelial cell by genistein. Using thiazolyl blue tetrazolium bromide(MTT) and [3H]-TdR assay, we found genistein inhibited angiotensin II-induced proliferation in primary cultured VSMC while it stimulated proliferation of quiescent endothelial cells. The effects were attenuated by caveolin1 or sprouty1 siRNA. Western blot analysis indicated that genistein attenuated the phosphorylation of extracellular regulated kinase1/2(ERK1/2) in angiotensin II-induced proliferated VSMC but stimulated the phosphorylation of ERK1/2 in quiescent endothelial cell. Double staining immunofluorescence identified caveolin1 and sprouty1 coexpressed in the cytoplasm of both VSMC and endothelial cell. Genistein increased the expression of caveolin1, p-caveolin1 and sprouty1 in VSMC, while it had opposite effects in quiescent endothelial cell. Co-immunoprecipitation suggested that genistein exerted its effects through interaction of caveolin1 and sprouty1. Our results demonstrate that the inhibition of angiotensin II-induced proliferation of VSMC and stimulation of quiescent endothelial cell by genistein are regulated by caveolin1 and sprouty1, which are implemented through Ras/MAPK pathway.
Authors:
QiuLing Xiang; GuiPing Lin; JinWen Xu; ShuHui Zheng; SiJuan Chen; KeWen Zhou; TingHuai Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-07
Journal Detail:
Title:  European journal of pharmacology     Volume:  648     ISSN:  1879-0712     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-10-12     Completed Date:  2011-01-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  153-61     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier B.V. All rights reserved.
Affiliation:
Department of Physiology, Zhongshan Medical School, Sun Yat-Sen University, 74 ZhongShan Rd 2, GuangZhou 510080, People's Republic of China.
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MeSH Terms
Descriptor/Qualifier:
Angiotensin II / pharmacology
Animals
Caveolin 1 / genetics,  metabolism*
Cell Proliferation / drug effects
Endothelial Cells / cytology*,  drug effects*,  metabolism
Female
Gene Expression Regulation, Enzymologic / drug effects
Genistein / pharmacology*
Mitogen-Activated Protein Kinase 1 / metabolism
Mitogen-Activated Protein Kinase 3 / metabolism
Muscle, Smooth, Vascular / cytology*,  drug effects*,  metabolism
Nerve Tissue Proteins / genetics,  metabolism*
Phosphorylation / drug effects
Phytoestrogens / pharmacology
Protein Transport / drug effects
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Caveolin 1; 0/Nerve Tissue Proteins; 0/Phytoestrogens; 0/Spry2 protein, rat; 11128-99-7/Angiotensin II; 446-72-0/Genistein; EC 2.7.11.24/Mitogen-Activated Protein Kinase 1; EC 2.7.11.24/Mitogen-Activated Protein Kinase 3

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