| The role of caspases in cryoinjury: caspase inhibition strongly improves the recovery of cryopreserved hematopoietic and other cells. | |
| | |
MedLine Citation:
|
PMID: 12207004 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Cryopreserved cells and tissues are increasingly used for stem cell transplantation and tissue engineering. However, their freezing, storage, and thawing is associated with severe damage, suggesting the need for better cryopreservation methods. Here, we show that activation of caspase-3 is induced during the freeze-thaw process. Moreover, we demonstrate that prevention of caspase activation by the caspase inhibitor zVAD-fmk strongly improves the recovery and survival of several cryopreserved cell types and hematopoietic progenitor cells. A short preincubation with the caspase inhibitor after thawing also enhances the colony-forming activity of hematopoietic progenitor cells up to threefold. Furthermore, overexpression of Bcl-2, but not the blockade of the death receptor signaling, confers protection, indicating that cryoinjury-associated cell death is mediated by a Bcl-2-controlled mitochondrial pathway. Thus, our data suggest the use of zVAD-fmk as an efficient cryoprotective agent. The addition of caspase inhibitors may be an important tool for the cryopreservation of living cells and advantageous in cell transplantation, tissue engineering, and other genetic technologies. |
| | |
Authors:
|
Christopher Stroh; Uwe Cassens; Ajoy K Samraj; Walter Sibrowski; Klaus Schulze-Osthoff; Marek Los |
Related Documents
:
|
16126354 - Isolation and viability of presumptive spermatids collected from bull testes by percoll... 6198664 - Fetal-to-adult hemopoietic cell transplantation: is hemoglobin synthesis gestational ag... 8050274 - Encapsulation of human erythrocytes by growing ice crystals. 18035754 - Improvement in rabbit corneal cell suspension viability after freezing with gingko bilo... 8678974 - Enzymatic properties of transplanted glomerulosa cells. 12743444 - New gastric epithelial cell lines from mice transgenic for temperature-sensitive simian... |
Publication Detail:
|
Type: Journal Article Date: 2002-08-07 |
Journal Detail:
|
Title: FASEB journal : official publication of the Federation of American Societies for Experimental Biology Volume: 16 ISSN: 1530-6860 ISO Abbreviation: FASEB J. Publication Date: 2002 Oct |
Date Detail:
|
Created Date: 2002-10-10 Completed Date: 2002-12-17 Revised Date: 2012-02-15 |
Medline Journal Info:
|
Nlm Unique ID: 8804484 Medline TA: FASEB J Country: United States |
Other Details:
|
Languages: eng Pagination: 1651-3 Citation Subset: IM |
Affiliation:
|
Institute of Experimental Dermatology, University of Münster, D-48149 Münster, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Amino Acid Chloromethyl Ketones
/
pharmacology* Animals Caspase 3 Caspases / antagonists & inhibitors*, physiology Cell Line Cell Survival / drug effects Cells, Cultured Cryopreservation / methods* Cysteine Proteinase Inhibitors / pharmacology* Freezing HeLa Cells Hematopoietic Stem Cells / cytology, drug effects Humans Jurkat Cells |
| Chemical | |
Reg. No./Substance:
|
0/Amino Acid Chloromethyl Ketones; 0/Cysteine Proteinase Inhibitors; 0/benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The relationship of hepatic tissue oxygenation with nitric oxide metabolism in ischemic precondition...
Next Document: Codon 12 and codon 13 mutations at the K-ras gene induce different soft tissue sarcoma types in nude...