Document Detail


The role of caspases in cryoinjury: caspase inhibition strongly improves the recovery of cryopreserved hematopoietic and other cells.
MedLine Citation:
PMID:  12207004     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cryopreserved cells and tissues are increasingly used for stem cell transplantation and tissue engineering. However, their freezing, storage, and thawing is associated with severe damage, suggesting the need for better cryopreservation methods. Here, we show that activation of caspase-3 is induced during the freeze-thaw process. Moreover, we demonstrate that prevention of caspase activation by the caspase inhibitor zVAD-fmk strongly improves the recovery and survival of several cryopreserved cell types and hematopoietic progenitor cells. A short preincubation with the caspase inhibitor after thawing also enhances the colony-forming activity of hematopoietic progenitor cells up to threefold. Furthermore, overexpression of Bcl-2, but not the blockade of the death receptor signaling, confers protection, indicating that cryoinjury-associated cell death is mediated by a Bcl-2-controlled mitochondrial pathway. Thus, our data suggest the use of zVAD-fmk as an efficient cryoprotective agent. The addition of caspase inhibitors may be an important tool for the cryopreservation of living cells and advantageous in cell transplantation, tissue engineering, and other genetic technologies.
Authors:
Christopher Stroh; Uwe Cassens; Ajoy K Samraj; Walter Sibrowski; Klaus Schulze-Osthoff; Marek Los
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Publication Detail:
Type:  Journal Article     Date:  2002-08-07
Journal Detail:
Title:  FASEB journal : official publication of the Federation of American Societies for Experimental Biology     Volume:  16     ISSN:  1530-6860     ISO Abbreviation:  FASEB J.     Publication Date:  2002 Oct 
Date Detail:
Created Date:  2002-10-10     Completed Date:  2002-12-17     Revised Date:  2012-02-15    
Medline Journal Info:
Nlm Unique ID:  8804484     Medline TA:  FASEB J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1651-3     Citation Subset:  IM    
Affiliation:
Institute of Experimental Dermatology, University of Münster, D-48149 Münster, Germany.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Chloromethyl Ketones / pharmacology*
Animals
Caspase 3
Caspases / antagonists & inhibitors*,  physiology
Cell Line
Cell Survival / drug effects
Cells, Cultured
Cryopreservation / methods*
Cysteine Proteinase Inhibitors / pharmacology*
Freezing
HeLa Cells
Hematopoietic Stem Cells / cytology,  drug effects
Humans
Jurkat Cells
Chemical
Reg. No./Substance:
0/Amino Acid Chloromethyl Ketones; 0/Cysteine Proteinase Inhibitors; 0/benzyloxycarbonylvalyl-alanyl-aspartyl fluoromethyl ketone; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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