Document Detail


The role of capsular polysaccharide K21b of Klebsiella and of the structurally related colanic-acid polysaccharide of Escherichia coli in resistance to phagocytosis and serum killing.
MedLine Citation:
PMID:  3320374     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The behaviour of strains of Klebsiella aerogenes of capsular serotype K21 and strains of Escherichia coli producing a structurally related polysaccharide (colanic acid) was analysed by phagocytic and serum-killing assays. The cell-surface characteristics of these strains and of non-capsulate strains derived from them were also investigated by partitioning experiments in aqueous two-polymer phase systems. The possession of K21-type capsule by K. aerogenes or colanic-acid polysaccharide by E. coli conferred a strong negative charge on capsulate bacteria. Negatively charged bacteria of E. coli producing colanic-acid capsules, however, like non-capsulate K. aerogenes, were susceptible to uptake by polymorphonuclear leukocytes. In contrast, K21 polysaccharide conferred on klebsiellae considerable resistance to phagocytic uptake. The finding that ingested non-capsulate derivative strains of K. aerogenes were less rapidly degraded by phagocytes than E. coli strains suggested that other components of the cell surface of Klebsiella, notably lipopolysaccharide, may be involved in protection against phagocytic killing. The presence of colanic-acid capsules on E. coli conferred little resistance to the bactericidal activity of human serum or phagocytic uptake and did not protect against intracellular killing by polymorphonuclear leukocytes.
Authors:
P M Allen; D Fisher; J R Saunders; C A Hart
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  24     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-02-09     Completed Date:  1988-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  363-70     Citation Subset:  IM    
Affiliation:
Department of Microbiology, University of Liverpool, London.
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MeSH Terms
Descriptor/Qualifier:
Blood Bactericidal Activity
Escherichia coli / immunology,  pathogenicity*,  ultrastructure
Klebsiella pneumoniae / immunology,  pathogenicity*,  ultrastructure
Microscopy, Electron
Neutrophils / physiology
Phagocytosis
Polysaccharides / biosynthesis,  physiology*
Polysaccharides, Bacterial / biosynthesis,  physiology*
Surface Properties
Virulence
Chemical
Reg. No./Substance:
0/Polysaccharides; 0/Polysaccharides, Bacterial; 9012-87-7/colanic acid

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