| The role of the calmodulin-dependent pathway in static magnetic field-induced mechanotransduction. | |
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MedLine Citation:
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PMID: 19953573 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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While the effects of static magnetic fields (SMFs) on osteoblastic differentiation are well demonstrated, the mechanotransduction pathways of SMFs are still unclear. The aim of this study was to explore the role of calmodulin in the biophysical effects of SMFs on osteoblastic cells. MG63 cells were exposed to a 0.4 T SMF. The expression of phosphodiesterase RNA in the cytoplasm was tested using real-time polymerase chain reaction. The differentiation of the cells was assessed by detecting changes in alkaline phosphatase activity. The role of calmodulin antagonist W-7 was used to evaluate alterations in osteoblastic proliferation and differentiation after the SMF simulations. Our results showed that SMF exposure increased alkaline phosphatase activity and phosphodiesterase 1C gene expression in MG63 cells. Addition of W-7 significantly inhibited the SMF-induced cellular response. We suggest that one possible mechanism by which SMFs affects osteoblastic maturation is through a calmodulin-dependent mechanotransduction pathway. |
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Authors:
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Jen-Chang Yang; Sheng-Yang Lee; Chi-An Chen; Che-Tong Lin; Chang-Chih Chen; Haw-Ming Huang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Bioelectromagnetics Volume: 31 ISSN: 1521-186X ISO Abbreviation: Bioelectromagnetics Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-04-14 Completed Date: 2010-07-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008281 Medline TA: Bioelectromagnetics Country: United States |
Other Details:
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Languages: eng Pagination: 255-61 Citation Subset: IM |
Affiliation:
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Graduate Institute of Biomedical Materials and Engineering, Taipei Medical University, Taipei, Taiwan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Calmodulin
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antagonists & inhibitors,
physiology* Cell Proliferation / drug effects, radiation effects Cells, Cultured Cyclic Nucleotide Phosphodiesterases, Type 1 / biosynthesis Electromagnetic Fields* Humans Mechanotransduction, Cellular* Osteoblasts / physiology, radiation effects* Sulfonamides / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Calmodulin; 0/Sulfonamides; 65595-90-6/W 7; EC 3.1.4.17/Cyclic Nucleotide Phosphodiesterases, Type 1; EC 3.1.4.17/PDE1C protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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