Document Detail

The role of calcium in the activation of glycogen phosphorylase in the fat body of the fruit beetle, Pachnoda sinuata, by hypertrehalosaemic hormone.
MedLine Citation:
PMID:  11341967     Owner:  NLM     Status:  MEDLINE    
The role of calcium in the mediation of the hypertrehalosaemic signal of the endogenous neuropeptide Mem-CC was investigated in vitro and in vivo in the cetoniid beetle Pachnoda sinuata. The presence of Mem-CC increases the influx of extracellular 45Ca(2+) into the fat body as well as the efflux of 45Ca(2+) from pre-loaded fat body into the incubation medium. Extracellular calcium is essential to exert maximal activation of the fat body glycogen phosphorylase by saturating doses of Mem-CC (0.3 nM). This effect of extracellular Ca(2+) is dose-dependent: maximal activation of glycogen phosphorylase by Mem-CC is achieved at calcium concentrations of approximately 1.2 mM and the ED(50) was calculated to be 0.6 mM. Both, thimerosal and thapsigargin caused a stimulation of carbohydrate metabolism in the fat body, suggesting that a release of calcium from the endoplasmic reticulum is involved in this process. However, neither entry of extracellular calcium nor the release from the endoplasmic reticulum are sufficient alone for a full activation of the phosphorylase. The results of the present study suggest that calcium from extracellular as well as from intracellular sources is part of the second messenger system for the transduction of the hypertrehalosaemic signal of Mem-CC in the fat body of P. sinuata.
L Auerswald; G Gäde
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1499     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-05-08     Completed Date:  2001-05-21     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  199-208     Citation Subset:  IM    
Zoology Department, University of Cape Town, 7701, Rondebosch, South Africa.
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MeSH Terms
Beetles / drug effects*,  metabolism*
Calcium / metabolism*,  pharmacology
Calcium Signaling
Endoplasmic Reticulum / drug effects,  metabolism
Enzyme Activation / drug effects
Fat Body / drug effects,  metabolism
Insect Hormones / pharmacology*
Models, Biological
Neuropeptides / pharmacology*
Nucleotides, Cyclic / pharmacology
Octopamine / pharmacology
Oligopeptides / pharmacology*
Phosphorylases / metabolism*
Pyrrolidonecarboxylic Acid / analogs & derivatives
Second Messenger Systems
Signal Transduction
Reg. No./Substance:
0/Insect Hormones; 0/Neuropeptides; 0/Nucleotides, Cyclic; 0/Oligopeptides; 0/hypertrehalosemic hormone; 104-14-3/Octopamine; 134599-16-9/adipokinetic hormone, beetle; 7440-70-2/Calcium; 98-79-3/Pyrrolidonecarboxylic Acid; EC 2.4.1.-/Phosphorylases

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