Document Detail


The role of c-myb during the maturation of murine CFU-E.
MedLine Citation:
PMID:  10349515     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proper expression of the c-myb proto-oncogene is essential for definitive, but not primitive erythropoiesis. To examine the role of c-myb during adult erythropoiesis, we incubated purified murine colony-forming units (CFU-E) with a c-myb-specific antisense oligodeoxynucleotide (AS-oligo) in order to diminish expression levels. By western blot analysis, c-myb expression was reduced during the first seven hours of AS-oligo treatment as compared to untreated cells. We then quantitated the amount of heme synthesized in CFU-E treated with c-myb AS-oligo, a random sequence oligo or no oligo. No significant differences were seen in the amount of heme synthesized during 42 hours of erythroid culture with either high levels (1 U/mL) or physiological levels (20 mU/mL) of Epo. In contrast, CFU-E treated with an AS-oligo directed toward mRNA encoding the first enzyme of the heme biosynthetic pathway in erythroid cells (d-aminolevulinate synthase) demonstrated a 65% reduction in the amount of heme synthesized. We conclude that the major role of c-myb during hematopoiesis must be in progenitor cells antecedent to the CFU-E stage and may possibly involve the establishment of the genetic program directing the formation of red blood cells.
Authors:
M M Gilmore; T R Bishop
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Blood cells, molecules & diseases     Volume:  25     ISSN:  1079-9796     ISO Abbreviation:  Blood Cells Mol. Dis.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-07-06     Completed Date:  1999-07-06     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9509932     Medline TA:  Blood Cells Mol Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  68-77     Citation Subset:  IM    
Affiliation:
Johns Hopkins University School of Medicine, Department of Pediatrics, Baltimore, Maryland 21205, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Differentiation / genetics
Erythroid Precursor Cells / cytology*,  metabolism*
Gene Expression Regulation, Developmental
Mice
Proto-Oncogene Proteins / genetics*
Proto-Oncogene Proteins c-myb
Trans-Activators / genetics*
Grant Support
ID/Acronym/Agency:
DK38052/DK/NIDDK NIH HHS; DK39888/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-myb; 0/Trans-Activators

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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