| A role for beta-sitosterol to stigmasterol conversion in plant-pathogen interactions. | |
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MedLine Citation:
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PMID: 20444228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Upon inoculation with pathogenic microbes, plants induce an array of metabolic changes that potentially contribute to induced resistance or even enhance susceptibility. When analysing leaf lipid composition during the Arabidopsis thaliana-Pseudomonas syringae interaction, we found that accumulation of the phytosterol stigmasterol is a significant plant metabolic process that occurs upon bacterial leaf infection. Stigmasterol is synthesized from beta-sitosterol by the cytochrome P450 CYP710A1 via C22 desaturation. Arabidopsis cyp710A1 mutant lines impaired in pathogen-inducible expression of the C22 desaturase and concomitant stigmasterol accumulation are more resistant to both avirulent and virulent P. syringae strains than wild-type plants, and exogenous application of stigmasterol attenuates this resistance phenotype. These data indicate that induced sterol desaturation in wild-type plants favours pathogen multiplication and plant susceptibility. Stigmasterol formation is triggered through perception of pathogen-associated molecular patterns such as flagellin and lipopolysaccharides, and through production of reactive oxygen species, but does not depend on the salicylic acid, jasmonic acid or ethylene defence pathways. Isolated microsomal and plasma membrane preparations exhibited a similar increase in the stigmasterol/beta-sitosterol ratio as whole-leaf extracts after leaf inoculation with P. syringae, indicating that the stigmasterol produced is incorporated into plant membranes. The increased contents of stigmasterol in leaves after pathogen attack do not influence salicylic acid-mediated defence signalling but attenuate pathogen-induced expression of the defence regulator flavin-dependent monooxygenase 1. P. syringae thus promotes plant disease susceptibility through stimulation of sterol C22 desaturation in leaves, which increases the stigmasterol to beta-sitosterol ratio in plant membranes. |
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Authors:
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Thomas Griebel; Jürgen Zeier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-04-26 |
Journal Detail:
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Title: The Plant journal : for cell and molecular biology Volume: 63 ISSN: 1365-313X ISO Abbreviation: Plant J. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-19 Completed Date: 2010-10-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9207397 Medline TA: Plant J Country: England |
Other Details:
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Languages: eng Pagination: 254-68 Citation Subset: IM |
Affiliation:
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Julius-von-Sachs-Institute of Biological Sciences, University of Würzburg, Würzburg, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arabidopsis
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genetics,
metabolism*,
microbiology Arabidopsis Proteins / genetics, metabolism Botrytis / pathogenicity Cytochrome P-450 Enzyme System / genetics, metabolism Gene Expression Regulation, Plant Host-Pathogen Interactions* Plant Diseases* / microbiology Plant Growth Regulators / metabolism Plant Leaves / metabolism, microbiology Pseudomonas syringae / pathogenicity Sitosterols / metabolism* Stigmasterol / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Arabidopsis Proteins; 0/Plant Growth Regulators; 0/Sitosterols; 5779-62-4/sitosterol; 83-48-7/Stigmasterol; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.14.-/CYP710A1 protein, Arabidopsis |
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