Document Detail

The role of beta-1,4-galactosyltransferase-I in the skin wound-healing process.
MedLine Citation:
PMID:  18212537     Owner:  NLM     Status:  MEDLINE    
Cell-surface carbohydrate chains are known to contribute to cell migration, interaction, and proliferation. beta-1,4-galactosyltransferase-I (beta-1,4-GalT-I), which is one of the best-studied glycosyltransferases, plays a key role in the synthesis of type 2 chains in N-glycans and the core 2 branch in O-glycans. Recently, it has been reported that skin wound healing is significantly delayed in beta-1,4-GalT-I mice. However, the expression of beta-1,4-GalT-I and its biological function in the skin wound-healing process remain to be elucidated. We used real-time polymerase chain reaction to demonstrate that the expression of beta-1,4-GalT-I mRNA reached plateau values at 12 hours after skin was injured and remained elevated until 11 days after the injury. Furthermore, lectin blotting showed that beta-1,4-galactosylated carbohydrate chains were also increased after skin injury. A double-staining method combining lectin-fluorescent staining with RCA-I and immunofluorescence was first used to determine the cellular localization of beta-1,4-galactosylated carbohydrate chains. Morphological analysis showed that the chains were primarily expressed in neutrophils and partially expressed in macrophages, endothelial cells, and collagen. Our results suggest that beta-1,4-GalT-I and beta-1,4-galactosylated carbohydrate chains participate in leukocyte recruitment, angiogenesis, and collagen deposition in the skin wound-healing process.
Aiguo Shen; Ji Qian; Lei Liu; Haiou Liu; Jianping Chen; Shuqiong Niu; Meijuan Yan; Xiaodong Chen; Congcong Shen; Jianxin Gu; Chun Cheng
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The American Journal of dermatopathology     Volume:  30     ISSN:  1533-0311     ISO Abbreviation:  Am J Dermatopathol     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-01-23     Completed Date:  2008-03-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7911005     Medline TA:  Am J Dermatopathol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  10-5     Citation Subset:  IM    
The Jiangsu Province Key Laboratory of Neural Regeneration, Nantong University, Nantong, People's Republic of China.
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MeSH Terms
Blotting, Western
Cell Membrane / chemistry,  metabolism
Chemotaxis, Leukocyte / physiology*
Collagen / metabolism
Fluorescent Antibody Technique
Galactosyltransferases / metabolism*
Gene Expression
Glycoproteins / chemistry,  metabolism
Neovascularization, Physiologic
RNA, Messenger / analysis
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Skin / enzymology*,  pathology
Wound Healing / physiology*
Reg. No./Substance:
0/Glycoproteins; 0/RNA, Messenger; 9007-34-5/Collagen; EC 2.4.1.-/Galactosyltransferases; EC 2.4.1.-/beta-1,4-galactosyltransferase I

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