Document Detail


The role of arachidonic acid metabolism in IL-3-induced proliferation.
MedLine Citation:
PMID:  3129485     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The role of arachidonic acid metabolites as second messengers in the IL-3-induced activation of DA-1 cells was examined. By using inhibitors of either the cyclooxygenase (CO) or lipoxygenase (LPO) pathways, we determined that neither prostaglandins nor leukotrienes were involved in signal transduction, since aspirin, indomethacin, meclofenamic acid, and nordihydroguaiaretic acid (NDGA) failed to inhibit the proliferation response of DA-1 cells to IL-3. Furthermore, two combination CO/LPO inhibitors, benoxaprofen and BW755c, failed to inhibit DA-1 proliferation. A new CO/LPO compound examined, SK&F 86002, did inhibit proliferation (IC50 = 30 microM +/- 14, N = 11), leading us to conclude this drug has other actions besides CO/LPO inhibition. Finally, direct measurement of 3H-arachidonic acid uptake by DA-1 cells failed to show a difference in the amount of 3H-arachidonic acid incorporated in the presence of limiting or saturating amounts of IL-3. We conclude from these data that arachidonic acid metabolites are not involved in transmembrane signalling by IL-3 in DA-1 cells.
Authors:
B E Barton; E WoldeMussie; L Wheeler
Related Documents :
21124055 - Purification and physiochemical characterization of melanin pigment from klebsiella sp....
3033625 - Studies on the effect of different inhibitors of arachidonic acid metabolism on glycery...
8865385 - Inositol phosphate turnover in human airways: effect of arachidonic acid metabolism.
3101515 - Ontogeny of 6-keto-pgf1 alpha synthesis in rabbit aorta and the effect of premature wea...
1691925 - Concentration gradients for hva, 5-hiaa, ascorbic acid, and uric acid in cerebrospinal ...
8048045 - Comparative teratology and transplacental pharmacokinetics of all-trans-retinoic acid, ...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Immunopharmacology and immunotoxicology     Volume:  10     ISSN:  0892-3973     ISO Abbreviation:  Immunopharmacol Immunotoxicol     Publication Date:  1988  
Date Detail:
Created Date:  1988-06-07     Completed Date:  1988-06-07     Revised Date:  2009-05-14    
Medline Journal Info:
Nlm Unique ID:  8800150     Medline TA:  Immunopharmacol Immunotoxicol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  35-52     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Allergan Inc., Irvine, CA 92715.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine
Anti-Inflammatory Agents, Non-Steroidal / pharmacology
Arachidonic Acids / metabolism*
Aspirin / pharmacology
Cell Division / drug effects
Cell Line / metabolism*
Indomethacin / pharmacology
Interleukin-3 / pharmacology*
Lipoxygenase Inhibitors
Meclofenamic Acid / pharmacology
Nordihydroguaiaretic Acid / pharmacology
Propionic Acids / pharmacology
Pyrazoles / pharmacology
Chemical
Reg. No./Substance:
0/Anti-Inflammatory Agents, Non-Steroidal; 0/Arachidonic Acids; 0/Interleukin-3; 0/Lipoxygenase Inhibitors; 0/Propionic Acids; 0/Pyrazoles; 50-78-2/Aspirin; 500-38-9/Nordihydroguaiaretic Acid; 51234-28-7/benoxaprofen; 53-86-1/Indomethacin; 644-62-2/Meclofenamic Acid; 66000-40-6/4,5-Dihydro-1-(3-(trifluoromethyl)phenyl)-1H-pyrazol-3-amine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Modulation of interleukin-1 activity on murine thymocytes by various inhibitors of arachidonic acid ...
Next Document:  Serum vitamin D metabolites do not change in response to intravenous injection of thyrotropin releas...