| The role of amino acids in corneal stromal healing: a method for evaluating cellular density and extracellular matrix distribution. | |
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MedLine Citation:
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PMID: 12699178 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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PURPOSE: To evaluate how amino acid supplementation influences the response of corneal stromal keratocytes and other components of the corneal stroma. METHODS: We compared two groups of patients undergoing cataract surgery. Each group included 20 eyes of 20 patients. Patients in Group 1 were treated with amino acids for 15 days before surgery, and patients in Group 2 did not receive amino acids (control). To evaluate differences and change in corneal stroma, we analyzed all of the operated corneas with confocal microscopy. The confocal images were then analyzed with a new method for measurement of corneal structure based on fractal dimension calculation. This led to a numerical value, D index (fractal surface dimension), an objective measurement of corneal stromal characteristics. This index is related to three factors: keratocyte density, stromal distribution pattern, and intercellular matrix structure. RESULTS: Patients in the preoperative amino acid group showed higher keratocyte density values than those in the control group. We observed differences in cell distribution patterns and intercellular matrix structures between groups. Amino acid-treated patients had a corneal stroma with a D index value 10% higher than control group patients. CONCLUSIONS: We hypothesized that preoperative oral supplements with amino acids may change the corneal stroma structure by increasing keratocyte density and modifying the intercellular matrix. A new method for the measurement of these changes was applied, which provides quantification of the stromal structure complexity with a single numerical value. |
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Authors:
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Ingrid Torres Munoz; Fabio Grizzi; Carlo Russo; Fabrizio I Camesasca; Nicola Dioguardi; Paolo Vinciguerra |
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Publication Detail:
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Type: Clinical Trial; Comparative Study; Controlled Clinical Trial; Journal Article |
Journal Detail:
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Title: Journal of refractive surgery (Thorofare, N.J. : 1995) Volume: 19 ISSN: 1081-597X ISO Abbreviation: J Refract Surg Publication Date: 2003 Mar-Apr |
Date Detail:
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Created Date: 2003-04-17 Completed Date: 2003-05-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9505927 Medline TA: J Refract Surg Country: United States |
Other Details:
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Languages: eng Pagination: S227-30 Citation Subset: IM |
Affiliation:
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Department of Ophthalmology, Istituto Clinico Humanitas, Milan, Italy. paolo.vinciguerra@humanitas.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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administration & dosage* Case-Control Studies Cataract Extraction / methods Cell Count Corneal Stroma / cytology*, surgery Dietary Supplements* Extracellular Matrix / metabolism* Fibroblasts / cytology Humans Image Processing, Computer-Assisted Microscopy, Confocal Preoperative Care / methods Random Allocation Wound Healing* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids |
| Comments/Corrections | |
Erratum In:
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J Refract Surg. 2003 May-Jun;19(3):288 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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