| The role of adenovirus early region 1A in the regulation of early regions 2A and 1B expression. | |
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MedLine Citation:
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PMID: 6316655 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The role of the adenovirus early region 1A (E1A) in the regulation of expression of early regions 2A (E2A) and 1B (E1B) has been investigated by microinjection of cloned segments of viral DNA into cultured cells. Plasmids carrying adenoviral sequences that code for the DNA binding protein (DBP) associated with either the early or the late E2A promoter (at map coordinates 75 and 72, respectively), or both promoters, were injected into cell nuclei in the presence or absence of a plasmid DNA containing early region 1A. The injected cells were scored for the expression of the DNA binding protein by indirect immunofluorescence using specific antisera. These studies suggest that the product(s) encoded by early region 1A differentially control the expression of the DNA binding protein. DBP produced from the early promoter is stimulated by addition of the E1A region. By contrast E1A gene product(s) inhibit the synthesis of the DBP species transcribed from the late promoter. It has been shown previously by a variety of studies that E1A regulates E1B expression. However, experiments in which E1B DNA was microinjected together with E1A and E2A suggest that the DNA binding protein may have a stimulatory effect on the synthesis of E1B products. |
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Authors:
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M Rossini |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Virology Volume: 131 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 1983 Nov |
Date Detail:
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Created Date: 1984-01-27 Completed Date: 1984-01-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 49-58 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviruses, Human
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genetics* Animals Base Sequence Cell Transformation, Viral* Cloning, Molecular Cricetinae DNA Restriction Enzymes DNA, Viral / genetics DNA-Binding Proteins / genetics Fibroblasts / enzymology Fluorescent Antibody Technique Genes, Viral* Kinetics Plasmids Thymidine Kinase / deficiency Transcription, Genetic |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/DNA-Binding Proteins; EC 2.7.1.21/Thymidine Kinase; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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