| The role of adenosine and ATP-sensitive potassium channels in the protection afforded by ischemic preconditioning against the post-ischemic endothelial dysfunction in guinea-pig hearts. | |
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MedLine Citation:
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PMID: 9769229 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The role of adenosine and ATP-sensitive potassium channels (KATP) in the mechanism of ischemic preconditioning (IPC)-induced protection against the post-ischemic endothelial dysfunction was studied. Langendorff-perfused guinea-pig hearts were subjected either to 40 min of global ischemia and 40 min reperfusion or were preconditioned prior to the ischemia/reperfusion with three cycles of either 5 min ischemia/5 min reperfusion (IPC) or 5 min infusion/5 min wash-out of adenosine, adenosine A1 receptor agonist, N6-cyclohexyladenosine (CHA) or KATP opener, pinacidil. The magnitude of coronary flow reduction caused by NO-synthase inhibitor, Nomega-nitro-l-arginine methyl ester (l-NAME), served as an index of a basal endothelium-dependent vasodilator tone. Coronary overflows produced by a bolus of acetylcholine (ACh) and sodium nitroprusside (SNP) were used as measures of agonist-induced endothelium-dependent and endothelium-independent vascular function, respectively. The coronary flow, LVDP, ACh response and l-NAME response were reduced by 8, 32, 41 and 54%, respectively, while SNP response was not changed in the hearts subjected to ischemia/reperfusion. ACh response was fully restored, l-NAME response was partially restored, and SNP response was not affected in the hearts subjected to IPC. The post-ischemic recoveries of coronary flow and LVDP were not improved by IPC. The protective effect of IPC on the ACh response was mimicked by adenosine, CHA, and pinacidil. The protective effect of IPC, CHA and pinacidil was abolished by KATP antagonist, glibenclamide. The IPC protection was affected neither by a non-specific adenosine antagonist, 8-p-sulfophenyltheophylline, nor by a specific adenosine A1 receptor antagonist, 8-cyclopentyl-1, 3-dipropylxanthine (DPCPX). Our data indicate that: (1) IPC affords endothelial protection in the mechanism that involves activation of KATP, but not adenosine A1 receptors; (2) exogenous adenosine and A1 receptor agonist afford the protection, which might be of a potential clinical significance; (3) the endothelial dysfunction is not involved in the mechanism of myocardial stunning in guinea-pig hearts. |
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Authors:
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M Maczewski; A Beresewicz |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of molecular and cellular cardiology Volume: 30 ISSN: 0022-2828 ISO Abbreviation: J. Mol. Cell. Cardiol. Publication Date: 1998 Sep |
Date Detail:
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Created Date: 1998-11-19 Completed Date: 1998-11-19 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0262322 Medline TA: J Mol Cell Cardiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1735-47 Citation Subset: IM |
Copyright Information:
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Copyright 1998 Academic Press. |
Affiliation:
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Department of Clinical Physiology, Medical Centre of Postgraduate Education, Warsaw, Poland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetylcholine
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pharmacology Adenosine / analogs & derivatives, pharmacology, physiology* Adenosine Triphosphate / physiology* Animals Endothelium, Vascular / drug effects, physiology* Enzyme Inhibitors / pharmacology Female Guinea Pigs Heart / drug effects, physiology* Hemodynamics Ischemic Preconditioning, Myocardial* Male NG-Nitroarginine Methyl Ester / pharmacology Nitroprusside / pharmacology Pinacidil / pharmacology Potassium Channel Blockers Potassium Channels / physiology* Vasodilator Agents / pharmacology Xanthines / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Potassium Channel Blockers; 0/Potassium Channels; 0/Vasodilator Agents; 0/Xanthines; 102146-07-6/1,3-dipropyl-8-cyclopentylxanthine; 15078-28-1/Nitroprusside; 36396-99-3/N(6)-cyclohexyladenosine; 50903-99-6/NG-Nitroarginine Methyl Ester; 51-84-3/Acetylcholine; 56-65-5/Adenosine Triphosphate; 58-61-7/Adenosine; 85371-64-8/Pinacidil |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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