Document Detail


The role of actin cables in directing the morphogenesis of the pharyngeal pouches.
MedLine Citation:
PMID:  14711875     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The pharyngeal arches are separated by endodermal outpocketings, the pharyngeal pouches. These are structures of considerable importance; they are required to segregate the mesenchymal populations of each arch and to induce the formation of arch components, and they generate specific derivatives, including the parathyroid and the thymus. The pharyngeal pouches are first evident as localised sites at which the endoderm contacts the ectoderm, and they then expand along the proximodistal axis to generate the narrow, tight morphology of the mature pouch. We currently have no knowledge of the morphogenetic mechanisms that direct formation of the pharyngeal pouches. Here, in chick, we show that cells within the pharyngeal pouch endoderm have an abundance of apically located actin fibres that are networked within the endodermal sheet, via their insertion into N-cadherin adherens junctions, to form a web of supra-cellular actin cables. Cytochalasin D disruption of these actin structures results in the formation of aberrant pouches that fail to generate their normal slit-like morphology. This suggests that the process of pharyngeal pouch morphogenesis involves the constraining influence of these actin cables that direct expansion, within the pouch, along the proximodistal axis. These results, importantly, provide us with vital insights into how the pharyngeal pouches form their normal morphology. They also give evidence, for the first time, of actin cables functioning as constraints during complex vertebrate morphogenetic episodes.
Authors:
Robyn Quinlan; Paul Martin; Anthony Graham
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-01-07
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  131     ISSN:  0950-1991     ISO Abbreviation:  Development     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-01-19     Completed Date:  2004-03-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  593-9     Citation Subset:  IM    
Affiliation:
MRC Centre for Developmental Neurobiology, 4th Floor New Hunts House, Guys Campus, Kings College London, London SE1 1UL, UK.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Animals
Body Patterning / drug effects,  physiology
Bone Morphogenetic Protein 7
Bone Morphogenetic Proteins / metabolism
Branchial Region / drug effects,  embryology*,  metabolism
Chick Embryo
Cytochalasin D / pharmacology
Nucleic Acid Synthesis Inhibitors / pharmacology
Transforming Growth Factor beta*
Chemical
Reg. No./Substance:
0/Actins; 0/Bone Morphogenetic Protein 7; 0/Bone Morphogenetic Proteins; 0/Nucleic Acid Synthesis Inhibitors; 0/Transforming Growth Factor beta; 22144-77-0/Cytochalasin D

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