| The role of UDP-glucose:hydroxycinnamate glucosyltransferases in phenylpropanoid metabolism and the response to UV-B radiation in Arabidopsis thaliana. | |
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MedLine Citation:
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PMID: 18563436 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Arabidopsis harbors four UDP-glycosyltransferases that convert hydroxycinnamates (HCAs) to 1-O-beta-glucose esters, UGT84A1 (encoded by At4g15480), UGT84A2 (At3g21560), UGT84A3 (At4g15490), and UGT84A4 (At4g15500). To elucidate the role of the individual UGT84A enzymes in planta we analyzed gene expression, UGT activities and accumulation of phenylpropanoids in Arabidopsis wild type plants, ugt mutants and overexpressing lines. Individual ugt84A null alleles did not significantly reduce the gross metabolic flux to the accumulating compounds sinapoylcholine (sinapine) in seeds and sinapoylmalate in leaves. For the ugt84A2 mutant, LC/MS analysis revealed minor qualitative and quantitative changes of several HCA choline esters and of disinapoylspermidine in seeds. Overexpression of individual UGT84A genes caused increased enzyme activities but failed to produce significant changes in the pattern of accumulating HCA esters. For UGT84A3, our data tentatively suggest an impact on cell wall-associated 4-coumarate. Exposure of plants to enhanced UV-B radiation induced the UGT84A-encoding genes and led to a transient increase in sinapoylglucose and sinapoylmalate concentrations. |
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Authors:
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Dirk Meissner; Andreas Albert; Christoph Böttcher; Dieter Strack; Carsten Milkowski |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-06-18 |
Journal Detail:
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Title: Planta Volume: 228 ISSN: 0032-0935 ISO Abbreviation: Planta Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-08-04 Completed Date: 2009-01-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1250576 Medline TA: Planta Country: Germany |
Other Details:
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Languages: eng Pagination: 663-74 Citation Subset: IM |
Affiliation:
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Department of Secondary Metabolism, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological* Arabidopsis / enzymology*, genetics, radiation effects Cell Wall Chromatography, High Pressure Liquid Cinnamates Gene Expression* / radiation effects Gene Expression Regulation, Plant Glucosyltransferases / metabolism* Glycosylation Malates / metabolism Mutation Phenylpropionates / metabolism Propanols / metabolism Reverse Transcriptase Polymerase Chain Reaction Transformation, Genetic Ultraviolet Rays |
| Chemical | |
Reg. No./Substance:
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0/1-phenylpropanol; 0/2-O-sinapoylmalate; 0/Cinnamates; 0/Malates; 0/Phenylpropionates; 0/Propanols; EC 2.4.1.-/Glucosyltransferases |
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