Document Detail


The role for T cell repertoire/antigen-specific interactions in experimental kidney ischemia reperfusion injury.
MedLine Citation:
PMID:  19561110     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
T cells have been implicated in the early pathogenesis of ischemia reperfusion injury (IRI) of kidney, liver, lung, and brain. It is not known whether Ag-TCR engagement followed by Ag-specific T cell activation participates in IRI. T cell-deficient nu/nu mice are moderately resistant to renal IRI, which can be reversed upon reconstitution with syngeneic T cells. In this study, we found that nu/nu mice reconstituted with DO11.10 T cells, limited in their TCR repertoire, have significantly less kidney dysfunction and tubular injury after renal IRI compared with that in nu/nu mice reconstituted with wild-type T cells having a diverse TCR repertoire. CD4(+) T cells infiltrating ischemic kidneys of nu/nu mice reconstituted with DO11.10 T cells exhibited lower IFN-gamma production than that of wild-type controls. Frequency of regulatory T cells in kidneys of these mice was similar in both DO11.10 T cells and wild-type T cell recipient groups. DO11.10 mice immunized with OVA-CFA had significantly worse kidney function at 24 h after ischemia than those immunized with CFA alone. Thus, without T cell activation, diverse TCR repertoire was important for renal IRI in naive mice. However, once T cells were activated in an Ag-specific manner through TCR in DO11.10 mice, a restricted TCR repertoire no longer limited the extent of kidney injury. Thus, both TCR repertoire-dependent and -independent factors mediate T cell functions in kidney IRI.
Authors:
Shailesh Ramchandra Satpute; Jong Myun Park; Hye Ryoun Jang; Patricia Agreda; Manchang Liu; Maria Teresa Gandolfo; Lorraine Racusen; Hamid Rabb
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2009-06-26
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  183     ISSN:  1550-6606     ISO Abbreviation:  J. Immunol.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-07     Completed Date:  2009-09-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  984-92     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine and Pathology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen Presentation
CD4-Positive T-Lymphocytes / physiology
Chemotaxis, Leukocyte
Epitopes, T-Lymphocyte / immunology*
Kidney Diseases / pathology*
Lymphocyte Activation / immunology
Mice
Mice, Nude
Reperfusion Injury / etiology,  immunology*
T-Lymphocytes / immunology*,  physiology,  transplantation
T-Lymphocytes, Regulatory / physiology
Grant Support
ID/Acronym/Agency:
R01 DK054770/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Epitopes, T-Lymphocyte

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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