Document Detail


On the role of the Shine-Dalgarno sequence in determining the efficiency of translation initiation at a weak start codon in the car operon of Escherichia coli K12.
MedLine Citation:
PMID:  2464695     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Translation of the carA gene is efficiently initiated at the intrinsically weak UUG codon. A single nucleotide substitution changing the Shine-Dalgarno box of carA (GGAGG) into the sequence TGAGG reduces translation of carA sevenfold. This result supports the view that extensive complementarity between the Shine-Dalgarno sequence and 16S RNA contributes significantly to the efficiency of translation when the latter starts at a weak initiation triplet.
Authors:
G Weyens; D Charlier; M Roovers; A Piérard; N Glansdorff
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of molecular biology     Volume:  204     ISSN:  0022-2836     ISO Abbreviation:  J. Mol. Biol.     Publication Date:  1988 Dec 
Date Detail:
Created Date:  1989-03-23     Completed Date:  1989-03-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985088R     Medline TA:  J Mol Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1045-8     Citation Subset:  IM    
Affiliation:
Erfelijkheidsleer en Microbiologie, Vrije Universiteit Brussel, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Codon*
Escherichia coli / genetics*
Genes, Bacterial
Lac Operon*
Molecular Sequence Data
Protein Biosynthesis*
RNA, Bacterial / genetics
RNA, Messenger*
RNA, Ribosomal, 16S / genetics
Chemical
Reg. No./Substance:
0/Codon; 0/RNA, Bacterial; 0/RNA, Messenger; 0/RNA, Ribosomal, 16S

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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