| The role of Ser54 in the antinociceptive activity of BmK9, a neurotoxin from the scorpion Buthus martensii Karsch. | |
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MedLine Citation:
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PMID: 21906612 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Residue 54 has been shown to be important for bioactivity in several toxins. However, its role in the antinociceptive activity of toxins has not been evaluated yet. In this study, site-directed mutagenesis and mouse acetic acid writhing test were used to investigate the role of Ser54 in the antinociceptive activity of BmK9 neurotoxin from the Buthus martensii Karsch scorpion. Detailed mutagenesis analysis revealed that substitution of Ser54 by various polar amino acids produced no significant change in the antinociceptive activity, while all substitutions of nonpolar amino acid for Ser54 led to a significant loss of antinociceptive activity. Following the conformational analysis, it was suggested that Ser54 in BmK9 plays a functional role in the antinociceptive activity, the residue exerts its effect by means of a side-chain hydrogen bond. |
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Authors:
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Yueqiu Wang; Zhihui Hao; Jianhua Shao; Yongbo Song; Chunli Li; Chuan Li; Yongshan Zhao; Yanfeng Liu; Tingting Wei; Chunfu Wu; Jinghai Zhang |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-8-31 |
Journal Detail:
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Title: Toxicon : official journal of the International Society on Toxinology Volume: - ISSN: 1879-3150 ISO Abbreviation: - Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-9-12 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1307333 Medline TA: Toxicon Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ltd. |
Affiliation:
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School of Life Science and Bio-pharmaceutics, Shenyang Pharmaceutical University, P.O. Box 17, 103. Wenhua Road, Shenhe District, Shenyang, Liaoning Province 110016, PR China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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