Document Detail


A role for Proline synthesis and transport in Listeria monocytogenes barotolerance.
MedLine Citation:
PMID:  21338448     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims:  To assess the contribution of proline biosynthesis to listerial barotolerance. Methods and Results:  Using a L. monocytogenes proBA deletion mutant, incapable of synthesizing proline, together with a proline overproducing strain, the contribution of proline synthesis to listerial barotolerance was determined. The ΔproBA strain does not survive as well as the wild-type when subjected to treatment of 500 MPa in rich media, and 400 MPa in minimal media (∼1 log lower survival in both conditions). Betaine and carnitine decrease the ability of the wild-type to survive at low pressures (300 MPa), but confer normal or slightly increased levels of protection at higher pressures (350 and 400 MPa). Conclusions:  A functional proline synthesis system is required for optimal survival of Listeria following treatment at high pressure levels (500 MPa in BHI and 400 MPa in DM), particularly where other compatible solutes are absent or limiting. Significance and impact of the Study:  Given the potential of high pressure processing as an effective food processing/safety strategy, understanding how pathogens such as Listeria have evolved to cope with such stresses is an important food safety consideration. In this context the work presented here may help to develop safer and more effective processing regimens.
Authors:
Kiera M Considine; Roy D Sleator; Alan L Kelly; Gerald F Fitzgerald; Colin Hill
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-2-22
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  -     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-2-22     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 The Society for Applied Microbiology.
Affiliation:
Department of MicrobiologyAlimentary Pharmabiotic CentreSchool of Food and Nutritional SciencesUniversity College Cork, Ireland and Department of Biological Sciences, Cork Institute of Technology, Ireland.
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