| The role of PDGF-BB on the development of the collateral circulation after acute arterial occlusion. | |
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MedLine Citation:
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PMID: 7803046 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The survival of tissues in the presence of arterial occlusion is critically dependent on the development of collateral blood vessels. Identification of the biochemical mediators and their mechanism of action is fundamental to an understanding of the evolution of the collateral circulation. The ability of PDGF-BB to promote this was evaluated in an animal model of hind limb ischaemia. In order to obtain significant quantities of this mitogen for use in our animal model, human recombinant PDGF-BB was expressed in a Chinese Hamster Ovary (CHO) cell line. The transfected CHO cells produced 544 micrograms/l of PDGF-BB in serum free medium (SFM). The 30 kDa form of PDGF-BB was purified to homogeneity as judged by silver staining and amino-acid sequencing. Purified PDGF-BB was shown to be bioactive by a cell proliferation assay. The exogenous administration of PDGF-BB enhanced the recovery of blood flow after acute arterial occlusion. The results suggest that PDGF-BB may have therapeutic value in promoting collateral development following arterial occlusion. |
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Authors:
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R N Martins; J O Chleboun; P Sellers; M Sleigh; J Muir |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Growth factors (Chur, Switzerland) Volume: 10 ISSN: 0897-7194 ISO Abbreviation: Growth Factors Publication Date: 1994 |
Date Detail:
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Created Date: 1995-01-30 Completed Date: 1995-01-30 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9000468 Medline TA: Growth Factors Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 299-306 Citation Subset: IM |
Affiliation:
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University Department of Surgery, Hollywood Private Hospital, Nedlands, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acute Disease Animals Arterial Occlusive Diseases / drug therapy*, physiopathology CHO Cells Collateral Circulation / drug effects*, physiology Cricetinae Disease Models, Animal Gene Expression Hindlimb / blood supply Humans Ischemia / drug therapy, physiopathology Male Platelet-Derived Growth Factor / genetics, pharmacology* Rats Rats, Wistar Recombinant Proteins / genetics, pharmacology Regional Blood Flow / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Platelet-Derived Growth Factor; 0/Recombinant Proteins; 0/platelet-derived growth factor BB |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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