| The role of L- and T-type Ca2+ currents during the in vitro aging of murine myogenic (i28) cells in culture. | |
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MedLine Citation:
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PMID: 17064763 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The age-related decline in skeletal muscle strength could, in part, result from alterations in the mechanism of excitation-contraction coupling, responsible for muscle contraction. In the present work, we used the in vitro aging of murine myogenic (i28) cells as a model, to investigate whether the inefficiency of aged satellite cells to generate functional skeletal muscle fibres could be partly due to defective voltage-dependent Ca2+ currents. The whole-cell patch clamp technique was employed to measure L- and T-type Ca2+ currents in myotubes derived from the differentiation and fusion of these cells reaching replicative senescence. Our data showed that the expression and the amplitude of these currents decreased significantly during in vitro aging. Moreover, the analysis of the L-type current evoked in young and old cells by positive voltage steps, revealed no differences in the kinetics of activation, but significant alterations in the rate of inactivation. These effects of in vitro aging on voltage-dependent Ca2+ currents could also be related to their inability to fuse into myotubes. Taken together, our data support the hypothesis that age-related effects on voltage-dependent L- and T-type currents could be one of the causes of the failure of satellite cells to efficiently counteract the impairment in muscle force. |
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Authors:
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Elisa Luin; Fabio Ruzzier |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-10-24 |
Journal Detail:
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Title: Cell calcium Volume: 41 ISSN: 0143-4160 ISO Abbreviation: Cell Calcium Publication Date: 2007 May |
Date Detail:
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Created Date: 2007-03-21 Completed Date: 2007-06-18 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8006226 Medline TA: Cell Calcium Country: Scotland |
Other Details:
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Languages: eng Pagination: 479-89 Citation Subset: IM |
Affiliation:
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Department of Physiology and Pathology and Centre for Neuroscience BRAIN, University of Trieste, Via A. Fleming 22, I-34127 Trieste, Italy. elisaluin@dfp.units.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcium / metabolism* Calcium Channels, L-Type / metabolism* Calcium Channels, T-Type / metabolism* Calcium Signaling* Cell Aging / physiology* Cell Count Cell Differentiation Cell Fusion Cell Nucleus / metabolism Cells, Cultured Electric Conductivity Ion Channel Gating Kinetics Mice Muscle Fibers, Skeletal / cytology*, metabolism* Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Calcium Channels, L-Type; 0/Calcium Channels, T-Type; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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