Document Detail


The role of IL-6 and IL-11 in hyperoxic injury in developing lung.
MedLine Citation:
PMID:  18214944     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We examined the cytoprotective effect of interleukin-6 (IL-6) and interleukin-11 (IL-11) during oxidant injury in neonatal lung and the regulators of cell death in vitro and in vivo after oxidant exposure. Type II cells from day 21 fetal neonatal rat lungs were treated with varying concentrations of either IL-6 or IL-11 for 24 hr prior to exposure to H(2)O(2). Three-day-old transgenic lung-specific IL-11 and IL-6 overexpressing and wild type (WT) mouse pups were exposed to hyperoxia or room air for 3 days. Type II cells exposed to either IL-6 or IL-11 prior to oxidant injury exhibited improved survival compared to controls, 67% +/- 2.6 survivals in IL-6 pretreated cells compared to 48% +/- 1.6 in control; 63% +/- 3 survivals in IL-11 pretreated cells compared to 49% +/- 2.6 in control. The number of TUNEL positive cells in hyperoxia-exposed lungs was increased compared to room air animals (27 +/- 0.9 vs. 4 +/- 0.4; mean +/- SEM; P < 0.05). In contrast, the number of TUNEL positive cells was reduced in hyperoxia-exposed lungs from IL-11 (+) mice (15.2 +/- 2.2; mean +/- SEM; P < 0.05). There was an enhanced accumulation of Bcl-2 and reduction of Bax protein in hyperoxia-exposed IL-11 (+) compared to room air-exposed mice. This was not seen in hyperoxia-exposed IL-6 (+) pups. An increase in caspase-3 was seen in hyperoxia-exposed lungs of WT pups compared to IL-11 (+) pups. IL-11 and IL-6 provide protective effects against oxidant-mediated injury in fetal type II cells and IL-11 provides protection in vivo by down-regulation of caspase-mediated cell death.
Authors:
Anne Chetty; Gong-Jee Cao; Nicholas Manzo; Heber C Nielsen; Aaron Waxman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Pediatric pulmonology     Volume:  43     ISSN:  1099-0496     ISO Abbreviation:  Pediatr. Pulmonol.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-04     Completed Date:  2008-04-01     Revised Date:  2010-06-16    
Medline Journal Info:
Nlm Unique ID:  8510590     Medline TA:  Pediatr Pulmonol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  297-304     Citation Subset:  IM    
Copyright Information:
(c) 2008 Wiley-Liss, Inc.
Affiliation:
Department of Pediatrics, Floating Hospital for Children, Tufts-New England Medical Center, Boston, Massachusetts 02111, USA. achetty@tufts-nemc.org
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Apoptosis
Bronchopulmonary Dysplasia / physiopathology*
Caspase 3 / immunology*
Cells, Cultured
Disease Models, Animal
Humans
Hyperoxia / immunology,  physiopathology*
In Situ Nick-End Labeling
Infant, Newborn
Interleukin-11 / physiology*
Interleukin-6 / physiology*
Lung / immunology,  physiology
Mice
Mice, Transgenic
Pulmonary Alveoli* / cytology,  immunology,  physiopathology
Rats
Grant Support
ID/Acronym/Agency:
HL 074859/HL/NHLBI NIH HHS; HL 37930/HL/NHLBI NIH HHS; HL 67089/HL/NHLBI NIH HHS; R01 HL037930-14/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Interleukin-11; 0/Interleukin-6; EC 3.4.22.-/Caspase 3

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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