| The role of IL-6 and IL-11 in hyperoxic injury in developing lung. | |
| | |
MedLine Citation:
|
PMID: 18214944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We examined the cytoprotective effect of interleukin-6 (IL-6) and interleukin-11 (IL-11) during oxidant injury in neonatal lung and the regulators of cell death in vitro and in vivo after oxidant exposure. Type II cells from day 21 fetal neonatal rat lungs were treated with varying concentrations of either IL-6 or IL-11 for 24 hr prior to exposure to H(2)O(2). Three-day-old transgenic lung-specific IL-11 and IL-6 overexpressing and wild type (WT) mouse pups were exposed to hyperoxia or room air for 3 days. Type II cells exposed to either IL-6 or IL-11 prior to oxidant injury exhibited improved survival compared to controls, 67% +/- 2.6 survivals in IL-6 pretreated cells compared to 48% +/- 1.6 in control; 63% +/- 3 survivals in IL-11 pretreated cells compared to 49% +/- 2.6 in control. The number of TUNEL positive cells in hyperoxia-exposed lungs was increased compared to room air animals (27 +/- 0.9 vs. 4 +/- 0.4; mean +/- SEM; P < 0.05). In contrast, the number of TUNEL positive cells was reduced in hyperoxia-exposed lungs from IL-11 (+) mice (15.2 +/- 2.2; mean +/- SEM; P < 0.05). There was an enhanced accumulation of Bcl-2 and reduction of Bax protein in hyperoxia-exposed IL-11 (+) compared to room air-exposed mice. This was not seen in hyperoxia-exposed IL-6 (+) pups. An increase in caspase-3 was seen in hyperoxia-exposed lungs of WT pups compared to IL-11 (+) pups. IL-11 and IL-6 provide protective effects against oxidant-mediated injury in fetal type II cells and IL-11 provides protection in vivo by down-regulation of caspase-mediated cell death. |
| | |
Authors:
|
Anne Chetty; Gong-Jee Cao; Nicholas Manzo; Heber C Nielsen; Aaron Waxman |
Related Documents
:
|
19406224 - Global transcriptional characterization of a mouse pulmonary epithelial cell line for u... 6893834 - Intracellular metabolism of the apoproteins of pulmonary surfactant in rat lung. 12397014 - Cystic fibrosis transmembrane conductance regulator modulates neurosecretory function i... 21954944 - Targeting tumour cells at the entrance. 4053034 - Flow cytometric analysis of the effect of sodium chloride on gastric cancer risk in the... 20164154 - Reactive oxygen species differently regulate renal tubular epithelial and interstitial ... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
|
Title: Pediatric pulmonology Volume: 43 ISSN: 1099-0496 ISO Abbreviation: Pediatr. Pulmonol. Publication Date: 2008 Mar |
Date Detail:
|
Created Date: 2008-02-04 Completed Date: 2008-04-01 Revised Date: 2010-06-16 |
Medline Journal Info:
|
Nlm Unique ID: 8510590 Medline TA: Pediatr Pulmonol Country: United States |
Other Details:
|
Languages: eng Pagination: 297-304 Citation Subset: IM |
Copyright Information:
|
(c) 2008 Wiley-Liss, Inc. |
Affiliation:
|
Department of Pediatrics, Floating Hospital for Children, Tufts-New England Medical Center, Boston, Massachusetts 02111, USA. achetty@tufts-nemc.org |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Animals, Newborn Apoptosis Bronchopulmonary Dysplasia / physiopathology* Caspase 3 / immunology* Cells, Cultured Disease Models, Animal Humans Hyperoxia / immunology, physiopathology* In Situ Nick-End Labeling Infant, Newborn Interleukin-11 / physiology* Interleukin-6 / physiology* Lung / immunology, physiology Mice Mice, Transgenic Pulmonary Alveoli* / cytology, immunology, physiopathology Rats |
| Grant Support | |
ID/Acronym/Agency:
|
HL 074859/HL/NHLBI NIH HHS; HL 37930/HL/NHLBI NIH HHS; HL 67089/HL/NHLBI NIH HHS; R01 HL037930-14/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Interleukin-11; 0/Interleukin-6; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effect of zinc supplementation on respiratory tract infections in children with cystic fibrosis.
Next Document: Finding consensus on the physiotherapy management of asymptomatic infants with cystic fibrosis.