| The role of IAP antagonist proteins in the core apoptosis pathway of the mosquito disease vector Aedes aegypti. | |
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MedLine Citation:
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PMID: 21274634 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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While apoptosis regulation has been studied extensively in Drosophila melanogaster, similar studies in other insects, including disease vectors, lag far behind. In D. melanogaster, the inhibitor of apoptosis (IAP) protein DIAP1 is the major negative regulator of caspases, while IAP antagonists induce apoptosis, in part, by binding to DIAP1 and inhibiting its ability to regulate caspases. In this study, we characterized the roles of two IAP antagonists, Michelob_x (Mx) and IMP, in apoptosis in the yellow fever mosquito Aedes aegypti. Overexpression of Mx or IMP caused apoptosis in A. aegypti Aag2 cells, while silencing expression of mx or imp attenuated apoptosis. Addition of recombinant Mx or IMP, but not cytochrome c, to Aag2 cytosolic extract caused caspase activation. Consistent with this finding, AeIAP1 bound and inhibited both initiator and effector caspases from A. aegypti, and Mx and IMP competed with caspases for binding to AeIAP1. However, a difference was observed in the BIR domains responsible for Dronc binding by AeIAP1 versus DIAP1. These findings demonstrate that the mechanisms by which IAP antagonists regulate apoptosis are largely conserved between A. aegypti and D. melanogaster, although subtle differences exist. |
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Authors:
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Hua Wang; Rollie J Clem |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Apoptosis : an international journal on programmed cell death Volume: 16 ISSN: 1573-675X ISO Abbreviation: Apoptosis Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-02-25 Completed Date: 2011-08-01 Revised Date: 2012-03-07 |
Medline Journal Info:
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Nlm Unique ID: 9712129 Medline TA: Apoptosis Country: United States |
Other Details:
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Languages: eng Pagination: 235-48 Citation Subset: IM |
Affiliation:
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Molecular, Cellular, and Developmental Biology Program, Arthropod Genomics Center, Division of Biology, Kansas State University, Manhattan, KS 66506, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aedes
/
cytology*,
drug effects,
enzymology,
metabolism* Animals Apoptosis* / drug effects Caspases / antagonists & inhibitors, metabolism Cell Extracts Cell Line Cytochromes c / metabolism Enzyme Activation / drug effects Enzyme Inhibitors / pharmacology Gene Silencing / drug effects Inhibitor of Apoptosis Proteins / antagonists & inhibitors*, metabolism Insect Proteins / metabolism* Insect Vectors / cytology, drug effects, enzymology, metabolism Protein Binding / drug effects Signal Transduction* / drug effects Sindbis Virus / drug effects, metabolism Yellow Fever / parasitology* |
| Grant Support | |
ID/Acronym/Agency:
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P20 RR16475/RR/NCRR NIH HHS; R21 AI067642/AI/NIAID NIH HHS; R21 AI067642-02/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Extracts; 0/Enzyme Inhibitors; 0/Inhibitor of Apoptosis Proteins; 0/Insect Proteins; 9007-43-6/Cytochromes c; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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