Document Detail

The role of a Drosophila POU homeo domain gene in the specification of neural precursor cell identity in the developing embryonic central nervous system.
MedLine Citation:
PMID:  8095484     Owner:  NLM     Status:  MEDLINE    
The Drosophila embryonic central nervous system (CNS) is derived from a stereotypic array of progenitor stem cells called neuroblasts (NBs). Each of the approximately 25 NBs per hemisegment undergoes repeated asymmetric divisions to produce, on average, 5-10 smaller ganglion mother cells (GMCs); each GMC, in turn, divides to produce two neurons. We demonstrate that the protein product encoded by a POU homeo domain gene (dPOU28/pdm-2) is expressed in the cell nuclei of a subset of NBs and GMCs. In the wild-type animal, GMC-1 is the only identified cell in the NB4-2 lineage that expresses dPOU28 protein to a high level, and it divides to produce the RP2 neuron and a second cell of unknown fate. Our results suggest that the presence of ectopically induced dPOU28/pdm-2 protein in the progeny of GMC-1 is sufficient to cause both of these cells to adopt their parental GMC-1 cell fate, leading to duplication of the RP2 neuron (and its sister cell) on the basis of both immunological and morphological criteria. These observations clearly implicate a role for dPOU28/pdm-2 in the specification of GMC-1 cell identity in the NB4-2 lineage and possibly in the specification of cell fate in other NB lineages in the developing embryonic CNS.
X Yang; S Yeo; T Dick; W Chia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genes & development     Volume:  7     ISSN:  0890-9369     ISO Abbreviation:  Genes Dev.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-04-13     Completed Date:  1993-04-13     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8711660     Medline TA:  Genes Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  504-16     Citation Subset:  IM    
Drosophila Neurobiology Laboratory, National University of Singapore.
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MeSH Terms
Animals, Genetically Modified
DNA Replication
Drosophila / embryology*,  genetics*
Embryo, Nonmammalian / physiology
Ganglia / embryology,  physiology*
Genes, Homeobox*
Heat-Shock Proteins / genetics
Nervous System / embryology*
Neurons / physiology*
Stem Cells / physiology
Transformation, Genetic
Reg. No./Substance:
0/Heat-Shock Proteins

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