| The role of a Drosophila POU homeo domain gene in the specification of neural precursor cell identity in the developing embryonic central nervous system. | |
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MedLine Citation:
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PMID: 8095484 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Drosophila embryonic central nervous system (CNS) is derived from a stereotypic array of progenitor stem cells called neuroblasts (NBs). Each of the approximately 25 NBs per hemisegment undergoes repeated asymmetric divisions to produce, on average, 5-10 smaller ganglion mother cells (GMCs); each GMC, in turn, divides to produce two neurons. We demonstrate that the protein product encoded by a POU homeo domain gene (dPOU28/pdm-2) is expressed in the cell nuclei of a subset of NBs and GMCs. In the wild-type animal, GMC-1 is the only identified cell in the NB4-2 lineage that expresses dPOU28 protein to a high level, and it divides to produce the RP2 neuron and a second cell of unknown fate. Our results suggest that the presence of ectopically induced dPOU28/pdm-2 protein in the progeny of GMC-1 is sufficient to cause both of these cells to adopt their parental GMC-1 cell fate, leading to duplication of the RP2 neuron (and its sister cell) on the basis of both immunological and morphological criteria. These observations clearly implicate a role for dPOU28/pdm-2 in the specification of GMC-1 cell identity in the NB4-2 lineage and possibly in the specification of cell fate in other NB lineages in the developing embryonic CNS. |
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Authors:
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X Yang; S Yeo; T Dick; W Chia |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Genes & development Volume: 7 ISSN: 0890-9369 ISO Abbreviation: Genes Dev. Publication Date: 1993 Mar |
Date Detail:
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Created Date: 1993-04-13 Completed Date: 1993-04-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8711660 Medline TA: Genes Dev Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 504-16 Citation Subset: IM |
Affiliation:
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Drosophila Neurobiology Laboratory, National University of Singapore. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Genetically Modified DNA Replication Drosophila / embryology*, genetics* Embryo, Nonmammalian / physiology Female Ganglia / embryology, physiology* Genes, Homeobox* Heat-Shock Proteins / genetics Immunohistochemistry Male Nervous System / embryology* Neurons / physiology* Stem Cells / physiology Transformation, Genetic |
| Chemical | |
Reg. No./Substance:
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0/Heat-Shock Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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