| The role of Desulfovibrio desulfuricans lipopolysaccharides in modulation of periodontal inflammation through stimulation of human gingival fibroblasts. | |
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MedLine Citation:
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PMID: 20627263 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVE: Periodontitis is a destructive disease which is likely to be the result of the activities of different microbial complexes. Recently, sulphate-reducing bacteria (SRB) have been detected in the oral cavity, and they have been found to be common inhabitants of sites showing periodontal destruction. The aim of study was to evaluate the influence of endotoxins of Desulfovibrio desulfuricans bacteria on human gingival fibroblast HGF-1 line. METHODS: The immunological response of gingival fibroblasts was evaluated by determination of their IL-6 and IL-8 secretion upon treatement with D. desulfuricans intestinal and type strain LPS, sodium butyrate (NaB) and IL-1beta. The amounts of cytokines were estimated by ELISA immunoassay. The influence of LPS and NaB on fibroblast proliferation was determined using the CyQUANT Cell Proliferation Assay Kit. RESULTS: No significant growth inhibition of cells exposed to LPS was observed, except for the culture growing in the presence of intestinal strain endotoxin at the highest concentration (100mug/ml). The secretion of IL-6 and IL-8 by fibroblasts was increased by D. desulfuricans endotoxins. Cells stimulated with proinflammatory cytokine 1L-1beta showed very high levels of both cytokines secretion. The release of IL-6 and IL-8 by cells in response to LPS and 1L-1beta was modulated by butyric acid. CONCLUSIONS: The observed response of gingival fibroblasts to stimulation by endotoxin suggests that D. desulfuricans can be involved in the pathogenesis of periodontitis. Moreover, butyrate present in the oral cavity seems to have immunoregulatory effect on cytokine production by gingival fibroblasts under physiological conditions and during microbe-induced inflammation. |
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Authors:
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Zofia Dzierżewicz; Joanna Szczerba; Jolanta Lodowska; Daniel Wolny; Arkadiusz Gruchlik; Arkadiusz Orchel; Ludmiła Węglarz |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2010-6-1 |
Journal Detail:
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Title: Archives of oral biology Volume: - ISSN: 1879-1506 ISO Abbreviation: - Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-7-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0116711 Medline TA: Arch Oral Biol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2010 Elsevier Ltd. All rights reserved. |
Affiliation:
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Department of Biopharmacy, Medical University of Silesia, Narcyzów St. 1, 41-200 Sosnowiec, Poland. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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