Document Detail

The role of BCL-2 and bax protein in monocyte-mediated apoptosis in human leukemic cell lines.
MedLine Citation:
PMID:  8950237     Owner:  NLM     Status:  MEDLINE    
Monocytes or monocyte-derived supernatants are able to kill leukemic cells via apoptosis, thereby preferentially effecting more mature leukemic cells. In the present study, the relationship between apoptosis and the apoptosis related proteins, bcl-2 and bax, was investigated in a number of human leukemic cell lines. Monocyte-derived supernatant induces extensive apoptosis in U937 myeloid leukemia cells and minor apoptosis in HL60 cells. No apoptosis was seen in four other cell lines (THP1, HL60-D3, KG1, and K562). The expression of bcl-2 and bax protein was determined in both groups of leukemic cell lines by flow cytometry (bcl-2 and bax) and Western blotting (bcl-2) at baseline level and after incubation with monocyte supernatant after different time periods. No clear relation was found between baseline bcl-2 or bax protein expression and the occurrence of apoptosis after incubation with monocyte supernatant. After different incubation time periods, no change was found in bcl-2 protein expression in U937 and K562 cells, whereas in KG1, HL60, and especially in THP1 cells, a significant decrease could be noticed. On the other hand, there was an increase in bcl-2 expression in HL60-D3 cells. Bax protein expression, measured at the same time points, remained essentially unchanged in HL60-D3 cells, decreased significantly in U937, HL60, and THP1 cells and slightly in K562 cells, and increased significantly in KG1 cells. Also, the ratio bax/bcl-2 decreased in HL60D3, but especially in U937 and HL60 cells, increased slightly in THP1 and KG1 cells, and remained essentially unchanged in K562 cells. Rh-tumor necrosis factor-alpha (TNF-alpha), the main mediator of monocyte mediated cytotoxicity, induced apoptosis in U937, HL60, and THP1 cells, thereby showing changes in bcl-2 expression similar to those found for monocyte derived supernatants. We concluded that in human leukemic cell lines, there is no relation between either bcl-2 or bax protein expression or the ratio of both, and apoptosis mediated by monocyte derived supernatant or TNF-alpha.
M C Legdeur; P M Bontje; G J Ossenkoppele; R H Beelen; A A van de Loosdrecht; M G Broekhoven; M M Langenhuijsen; S F Thijsen; H Hofstee; G J Schuurhuis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental hematology     Volume:  24     ISSN:  0301-472X     ISO Abbreviation:  Exp. Hematol.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1996-12-26     Completed Date:  1996-12-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0402313     Medline TA:  Exp Hematol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1530-9     Citation Subset:  IM    
Department of Hematology, Free University Hospital, Amsterdam, The Netherlands.
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MeSH Terms
Apoptosis / drug effects,  physiology*
Blotting, Western
Flow Cytometry
Fluorescent Antibody Technique, Indirect
Leukemia / pathology*
Monocytes / physiology*
Proto-Oncogene Proteins / biosynthesis,  physiology*
Proto-Oncogene Proteins c-bcl-2 / biosynthesis,  physiology*
Tumor Cells, Cultured
bcl-2-Associated X Protein
Reg. No./Substance:
0/BAX protein, human; 0/Proto-Oncogene Proteins; 0/Proto-Oncogene Proteins c-bcl-2; 0/bcl-2-Associated X Protein

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