Document Detail

A role for ADP ribosylation factor in the control of cargo uptake during COPI-coated vesicle biogenesis.
MedLine Citation:
PMID:  10622709     Owner:  NLM     Status:  MEDLINE    
ARF-mediated hydrolysis of GTP has been demonstrated to regulate coat disassembly of Golgi-derived COPI transport vesicles (Tanigawa, G., Orci, L., Amherdt, M., Ravazzola, M., Helms, J.B. and Rothman, J.E. (1993) J. Cell Biol. 123, 1365-1371). In addition, a requirement for GTP hydrolysis at an early stage of COPI vesicle biogenesis has been established since cargo uptake is impaired in the presence of GTPgammaS (Nickel, W., Malsam, J., Gorgas, K., Ravazzola, M., Jenne, N., Helms, J.B. and Wieland, F.T. (1998) J. Cell Sci. 111, 3081-3090), a non-hydrolyzable analogue of GTP. We now demonstrate that the GTPase involved in the regulation of cargo uptake is ARF, revealing a multi-functional role of this GTPase in COPI-mediated vesicular transport. The molecular mechanism of cargo uptake as well as the functional implications of these findings on the overall process of COPI vesicle biogenesis are discussed.
J Malsam; D Gommel; F T Wieland; W Nickel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  462     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-02-02     Completed Date:  2000-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  267-72     Citation Subset:  IM    
Biochemie Zentrum Heidelberg, Ruprecht Karls-Universität Heidelberg, Germany.
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MeSH Terms
ADP-Ribosylation Factors / physiology*
CHO Cells
Cell Line
Coat Protein Complex I / metabolism*
Coated Vesicles / metabolism*
Golgi Apparatus / metabolism
Guanosine 5'-O-(3-Thiotriphosphate) / metabolism
Intracellular Membranes / metabolism
Reg. No./Substance:
0/Coat Protein Complex I; 37589-80-3/Guanosine 5'-O-(3-Thiotriphosphate); EC Factors

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