Document Detail

A role for 3-O-sulfotransferase isoform-4 in assisting HSV-1 entry and spread.
MedLine Citation:
PMID:  16259945     Owner:  NLM     Status:  MEDLINE    
Many heparan sulfate (HS) 3-O-sulfotransferase (3-OST) isoforms generate cellular receptors for herpes simplex virus type-1 (HSV-1) glycoprotein D (gD). Interestingly, the ability of 3-OST-4 to mediate HSV-1 entry and cell-to-cell fusion has not been determined, although it is predominantly expressed in the brain, a primary target of HSV-1 infections. We report that expression of 3-OST-4 can render Chinese hamster ovary K1 (CHO-K1) cells susceptible to entry of wild-type and a mutant (Rid1) strain of HSV-1. Evidence for generation of gD receptors by 3-OST-4 was suggested by gD-mediated interference assay and the ability of 3-OST-4 expressing CHO-K1 cells to preferentially bind HSV-1 gD, which could be reversed by prior treatment of cells with HS lyases (heparinases-II/III). In addition, 3-OST-4 expressing CHO-K1 cells acquired the ability to fuse with cells-expressing HSV-1 glycoproteins. Demonstrating specificity, the cell fusion was inhibited by soluble 3-O-sulfated forms of HS, but not unmodified HS. Taken together our results suggest a role of 3-OST-4 in HSV-1 pathogenesis.
Vaibhav Tiwari; Christopher D O'Donnell; Myung-Jin Oh; Tibor Valyi-Nagy; Deepak Shukla
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2005-10-21
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  338     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  2005 Dec 
Date Detail:
Created Date:  2005-11-15     Completed Date:  2006-01-12     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  930-7     Citation Subset:  IM    
Department of Ophthalmology and Visual Sciences, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
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MeSH Terms
CHO Cells
Herpesvirus 1, Human / physiology*
Membrane Fusion / physiology*
Protein Isoforms / metabolism
Sulfotransferases / metabolism*
Virus Replication / physiology*
Grant Support
Reg. No./Substance:
0/Protein Isoforms; EC 2.8.2.-/Sulfotransferases; EC sulfotransferase

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