Document Detail


The rolB gene suppresses reactive oxygen species in transformed plant cells through the sustained activation of antioxidant defense.
MedLine Citation:
PMID:  22271748     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The rolB (for rooting locus of Agrobacterium rhizogenes) oncogene has previously been identified as a key player in the formation of hairy roots during the plant-A. rhizogenes interaction. In this study, using single-cell assays based on confocal microscopy, we demonstrated reduced levels of reactive oxygen species (ROS) in rolB-expressing Rubia cordifolia, Panax ginseng, and Arabidopsis (Arabidopsis thaliana) cells. The expression of rolB was sufficient to inhibit excessive elevations of ROS induced by paraquat, menadione, and light stress and prevent cell death induced by chronic oxidative stress. In rolB-expressing cells, we detected the enhanced expression of antioxidant genes encoding cytosolic ascorbate peroxidase, catalase, and superoxide dismutase. We conclude that, similar to pathogenic determinants in other pathogenic bacteria, rolB suppresses ROS and plays a role not only in cell differentiation but also in ROS metabolism.
Authors:
Victor P Bulgakov; Tatiana Y Gorpenchenko; Galina N Veremeichik; Yuri N Shkryl; Galina K Tchernoded; Dmitry V Bulgakov; Dmitry L Aminin; Yuri N Zhuravlev
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-01-23
Journal Detail:
Title:  Plant physiology     Volume:  158     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-03-07     Completed Date:  2012-06-21     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1371-81     Citation Subset:  IM    
Affiliation:
Institute of Biology and Soil Science, Far East Branch of Russian Academy of Sciences, Vladivostok 690022, Russia. bulgakov@ibss.dvo.ru
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MeSH Terms
Descriptor/Qualifier:
Agrobacterium / genetics*
Antioxidants / metabolism*
Arabidopsis / cytology,  drug effects,  genetics,  metabolism
Ascorbate Peroxidases / genetics,  metabolism
Bacterial Proteins / genetics,  metabolism*
Cell Death
Cell Survival
Culture Media / metabolism
Glutathione / metabolism
Light
Oxidative Stress
Panax / cytology,  drug effects,  genetics,  metabolism
Paraquat / pharmacology
Plant Cells / drug effects,  metabolism*
Plants, Genetically Modified / cytology,  genetics,  metabolism
Reactive Oxygen Species / metabolism*
Rubia / drug effects,  genetics,  metabolism
Salt-Tolerant Plants / cytology,  drug effects,  genetics,  metabolism
Sodium Chloride / pharmacology
Superoxide Dismutase / genetics,  metabolism
Vitamin K 3 / pharmacology
beta-Glucosidase / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Bacterial Proteins; 0/Culture Media; 0/Reactive Oxygen Species; 4685-14-7/Paraquat; 58-27-5/Vitamin K 3; 70-18-8/Glutathione; 7647-14-5/Sodium Chloride; EC 1.11.1.11/Ascorbate Peroxidases; EC 1.15.1.1/Superoxide Dismutase; EC 3.2.1.-/RolB protein, Agrobacterium rhizogenes; EC 3.2.1.21/beta-Glucosidase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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