Document Detail


A robust microfluidic in vitro cell perifusion system.
MedLine Citation:
PMID:  22256299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present here a robust microfluidic cell perifusion device for in vitro primary tissue cell secretion studies. This system increases the sample concentration to perifusion volume ratio by an order of magnitude compared with standard multi-well plate static incubation assays. Further, this device achieves physiologically relevant flow rates, pressures, and temperature. It has been manufactured with typical machining facilities, principally drilling and milling. No specialist clean room equipment is required to replicate it. We show its capability here with hormone perifusion experiments on primary pancreatic tissue from mice. This device can increase cell secretion concentrations by up to a factor of 20, allowing for the first time the direct measurement of islet glucagon using mass spectrometry.
Authors:
Christina Morris; Dylan J Banks; Lukasz Gaweda; Steve Scott; Xi Xi Zhu; Maria Panico; Pantelis Georgiou; Christofer Toumazou
Related Documents :
12056559 - Regulation of nociceptive neurons by nerve growth factor and glial cell line derived ne...
15312169 - Induction of pc12 cell differentiation by flavonoids is dependent upon extracellular si...
16144659 - Differential effect of calmodulin antagonists on mg132-induced mitochondrial dysfunctio...
2253329 - Hippocampal neurotrophic factors influence the perikaryal size of septal acetylcholines...
11129979 - New structural analogues of tubulozole induce apoptosis, [ca2+]i modifications and cyto...
4065219 - Nerve growth factor and glucocorticoids regulate phenotypic expression in cultured chro...
10612539 - B-cell leukemia protein-2 and peptide yy chemotherapy resistance in colon cancer.
11541279 - Cell wall elasticity: i. a critique of the bulk elastic modulus approach and an analysi...
16660529 - Structure of plant cell walls: viii. a new pectic polysaccharide.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference     Volume:  2011     ISSN:  1557-170X     ISO Abbreviation:  Conf Proc IEEE Eng Med Biol Soc     Publication Date:  2011  
Date Detail:
Created Date:  2012-01-18     Completed Date:  2012-05-31     Revised Date:  2014-08-21    
Medline Journal Info:
Nlm Unique ID:  101243413     Medline TA:  Conf Proc IEEE Eng Med Biol Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8412-5     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Biological Assay
Female
Glucagon / secretion
Glucose / pharmacology
Islets of Langerhans / drug effects,  metabolism
Mice
Microfluidics / methods*
Perfusion / methods*
Chemical
Reg. No./Substance:
9007-92-5/Glucagon; IY9XDZ35W2/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Towards a cellular multi-parameter analysis platform: fluorescence in situ hybridization (FISH) on m...
Next Document:  Optimization for enhancement of signal effectiveness in three-dimensional (3D) cell based electroche...