Document Detail


Ser/Thr-rich domains are associated with genetic variation and morphogenesis in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  16823884     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Proteins containing regions of amino acid bias are often found in eukaryotes and are associated with particular functional groups. We have carried out a genomic analysis of yeast proteins containing regions with a significant bias of Ser and Thr residues. Our findings reveal that a high number are cell surface proteins or regulatory proteins involved in many aspects of cell differentiation. Furthermore, in Saccharomyces-related species, a highly significant correlation exists between the frequency of Ser-rich regions and DNA repeats, indicating that their generation may rely on similar factors. Cluster analysis shows that Ser/Thr-rich regions, located within the tandem repeats of cell surface proteins, are encoded to an increased frequency by UCU (Ser) and ACU/ACC (Thr), implying that mutational events that generate iterations could involve these codons. Replication slippage is proposed to be a contributing factor, as mounting evidence suggests that repeat generation in cell surface proteins can occur independently of meiosis. To reinforce this argument, we have discovered a premeiotic association between Mre11p, a nuclease involved in DNA repair, and ORFs encoding Ser/Thr-rich regions. Several macromolecules involved in the glycosylation and phosphorylation of proteins require Ser and Thr residues as binding sites. Ser/Thr-rich regions, through polymorphisms, are associated with the evolution of functional sites, particularly in providing motifs for glycosylation and phosphorylation. These results point to a Ser/Thr-biased somatic mutation mechanism that contributes to rapid evolution in yeast.
Authors:
Suzanne Bowen; Alan E Wheals
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Yeast (Chichester, England)     Volume:  23     ISSN:  0749-503X     ISO Abbreviation:  Yeast     Publication Date:  2006 Jun 
Date Detail:
Created Date:  2006-07-12     Completed Date:  2006-09-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8607637     Medline TA:  Yeast     Country:  England    
Other Details:
Languages:  eng     Pagination:  633-40     Citation Subset:  IM    
Copyright Information:
Copyright 2006 John Wiley & Sons, Ltd.
Affiliation:
Department of Biology and Biochemistry, University of Bath, Bath BA2 7AY, UK.
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MeSH Terms
Descriptor/Qualifier:
Cluster Analysis
Genetic Variation
Genome, Fungal / genetics*
Open Reading Frames
Protein Structure, Tertiary
Protein-Serine-Threonine Kinases / chemistry,  genetics*
Saccharomyces cerevisiae / enzymology,  genetics*,  growth & development
Saccharomyces cerevisiae Proteins / chemistry,  genetics*
Serine / genetics*
Tandem Repeat Sequences
Threonine / genetics*
Chemical
Reg. No./Substance:
0/Saccharomyces cerevisiae Proteins; 56-45-1/Serine; 72-19-5/Threonine; EC 2.7.11.1/Protein-Serine-Threonine Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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