Document Detail


rhomboid mediates specification of blue- and green-sensitive R8 photoreceptor cells in Drosophila.
MedLine Citation:
PMID:  19261861     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Color vision is based on the differential color sensitivity of retinal photoreceptors, however the developmental programs that control photoreceptor cell differentiation and specify color sensitivity are poorly understood. In Drosophila there is growing evidence that the color sensitivity of the R8 cell within an individual ommatidium is regulated by an inductive signal from the adjacent R7 cell. We previously examined the retinal patterning defect in Scutoid mutants, which results from a disruption of rhomboid expression. Here we show that loss of rhomboid blocks the induction of Rh5 expression and misexpression of rhomboid leads to the inappropriate induction of Rh5. These effects are specific to rhomboid, because its paralogue roughoid is neither required nor sufficient for the induction of Rh5 expression. We show that rhomboid is required cell-autonomously within the R8 photoreceptor cells and nonautonomously elsewhere in the eye for Rh5 induction. Interestingly, we found that the Epidermal growth factor receptor is also required for Rh5 induction, and its activation is sufficient to rescue the loss of Rh5 induction in a rhomboid mutant. This suggests that rhomboid may function in R8 cells to activate Epidermal growth factor receptor signaling in R7 cells and promote their differentiation to a signaling competent state.
Authors:
Denise A Birkholz; Wen-Hai Chou; Meridee M Phistry; Steven G Britt
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  29     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-03-05     Completed Date:  2009-04-10     Revised Date:  2014-09-15    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2666-75     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Animals
Cell Differentiation / genetics,  physiology
Compound Eye, Arthropod / anatomy & histology,  metabolism,  physiology
Drosophila
Drosophila Proteins / biosynthesis,  genetics,  physiology*
Genotype
Immunohistochemistry
Membrane Proteins / genetics,  physiology*
Microscopy, Electron, Scanning
Photoreceptor Cells, Invertebrate / cytology*,  physiology*
Receptor, Epidermal Growth Factor / physiology
Rhodopsin / biosynthesis,  genetics
rho-Associated Kinases / genetics
Grant Support
ID/Acronym/Agency:
EYR01-12423//PHS HHS; R01 EY012423/EY/NEI NIH HHS; R01 EY012423-05/EY/NEI NIH HHS; R01 EY018376/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Drosophila Proteins; 0/Membrane Proteins; 0/Rh5 protein, Drosophila; 0/Rho protein, Drosophila; 9009-81-8/Rhodopsin; EC 2.7.10.1/Receptor, Epidermal Growth Factor; EC 2.7.11.1/rho-Associated Kinases
Comments/Corrections

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