Document Detail


A review of folate receptor alpha cycling and 5-methyltetrahydrofolate accumulation with an emphasis on cell models in vitro.
MedLine Citation:
PMID:  15094208     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Folate receptor alpha (FRalpha), a glycosyl phosphatidylinositol linked protein with a great affinity for folic acid and some reduced folates such as 5-methyltetrahydrofolate and tetrahydrofolate is present on a limited number of epithelial cells, especially the kidney, placenta and choroid plexus. It is also over-expressed in many carcinomas. The receptor appears to remain membrane bound but cycles between the cell surface and an internal compartment. Its localization to detergent resistant membranes (lipid rafts) may be important to its cycling and indeed putative function to conserve folate in selected body compartments. Agents that disrupt the actin cytoskeleton such as cytochalasin D as well as phorbol myristic acid effect cycling of the receptor. The monkey kidney cell line, MA104, has proved to be a useful model for studying FRalpha cycling and 5-methyltetrahydrofolate accumulation. This chapter reviews much of this work and compares and contrasts it to studies of other cells, both normal and malignant.
Authors:
Barton A Kamen; Angel K Smith
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Advanced drug delivery reviews     Volume:  56     ISSN:  0169-409X     ISO Abbreviation:  Adv. Drug Deliv. Rev.     Publication Date:  2004 Apr 
Date Detail:
Created Date:  2004-04-19     Completed Date:  2004-11-16     Revised Date:  2005-11-16    
Medline Journal Info:
Nlm Unique ID:  8710523     Medline TA:  Adv Drug Deliv Rev     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1085-97     Citation Subset:  IM    
Affiliation:
Cancer Institute of New Jersey, Robert Wood Johnson Medical School, 195 Little Albany Street, New Brunswick, NJ 80901, USA. kamenba@umdnj.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Transport / physiology
Carrier Proteins / chemistry,  metabolism*
Cell Line
Cytoplasm / metabolism*
Folic Acid / chemistry*,  metabolism*
Humans
Receptors, Cell Surface / chemistry,  metabolism*
Tetrahydrofolates / metabolism*
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Receptors, Cell Surface; 0/Tetrahydrofolates; 0/folate-binding protein; 134-35-0/5-methyltetrahydrofolate; 59-30-3/Folic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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