Document Detail


The reversibility of impaired prostacyclin production of the vein graft.
MedLine Citation:
PMID:  8412121     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of changes in shear stress (shear stress variation) on production of prostacyclin (PGI2) were examined in canine autologous vein grafts, which were implanted in the poor or normal distal runoff limbs. Four weeks after grafting, the vein grafts were perfused ex vivo and PGI2 was assayed as 6-ketoprostaglandin F1 alpha. The vein grafts were perfused under constant flow for the first 30 min and then under pulsatile flow simulating an abnormal flow with a low shear stress variation or a normal flow with a high shear stress variation for the next 30 min. Basal production rates (30-min cumulative PGI2 production) of vein grafts implanted in the poor runoff and normal runoff limbs were 1.97 +/- 0.71 and 2.19 +/- 0.40 ng/cm2, respectively, with no significant difference. Pulsatile flow effects (increased PGI2 production between 30 and 60 min) of simulated abnormal versus normal flow were 0.50 +/- 0.50 ng/cm2 versus 2.31 +/- 1.87 ng/cm2 in vein grafts implanted in poor runoff limbs (P < 0.05) and 0.48 +/- 0.41 ng/cm2 versus 3.48 +/- 1.08 ng/cm2 in vein grafts implanted in normal runoff limbs (P < 0.01), respectively. There were no significant differences in simulated normal flow effects between poor runoff limbs and normal runoff limbs. The results indicate that the release of PGI2 in vein grafts may decrease in the presence of an abnormal blood flow with a low shear stress variation. In addition, it is suggested that even vein grafts implanted in the poor runoff could increase PGI2 production once the grafts were placed into normal arterial circulation.
Authors:
T Onohara; K Okadome; T Ishii; S Yamamura; K Komori; K Sugimachi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of surgical research     Volume:  55     ISSN:  0022-4804     ISO Abbreviation:  J. Surg. Res.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-11-04     Completed Date:  1993-11-04     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0376340     Medline TA:  J Surg Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  344-50     Citation Subset:  IM    
Affiliation:
Second Department of Surgery, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Flow Velocity
Dogs
Endothelium, Vascular / ultrastructure
Epoprostenol / biosynthesis*
Female
Male
Microscopy, Electron, Scanning
Perfusion
Rheology
Veins / physiology,  transplantation*,  ultrastructure
Chemical
Reg. No./Substance:
35121-78-9/Epoprostenol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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