Document Detail


A reverse transcription-polymerase chain reaction bioassay for avian vitellogenin mRNA.
MedLine Citation:
PMID:  11714249     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A bioassay based on the measurement of vitellogenin (VTG) mRNA in avian embryo hepatocyte cultures by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) was developed. To allow sequence comparison and design of suitable PCR primers, a short region of VTG cDNA was cloned and sequenced for seven species of birds. Cell cultures were prepared from both chicken and herring gull embryos and treated with the estradiol analogue moxestrol or the organochlorine insecticide o,p'-DDT. Using primers based on an area of the VTG gene that was identical for herring gull and chicken, in vitro VTG mRNA induction was observed for both moxestrol- and o,p'-DDT-treated cultures. Herring gull embryo hepatocyte cultures responded with VTG mRNA induction at moxestrol concentrations of 1 nM compared with 10 nM in chicken embryo hepatocyte cultures. Both herring gull and chicken embryo hepatocyte cultures responded with substantial VTG mRNA induction when treated with 10,000 nM o,p'-DDT. These results suggest that the bioassay will be useful for comparing avian embryo hepatocyte culture concentration-response data in terms of intra- and interspecies sensitivities to pharmacological estrogens or environmental contaminants.
Authors:
A Lorenzen; W L Casley; T W Moon
Related Documents :
21854389 - Blood-brain barrier modeling with co-cultured neural progenitor cell-derived astrocytes...
3374279 - Lipogenic enzyme activities in primary cultures of adult mouse hepatocytes.
3056629 - Cell density dependent morphological changes in adult rat hepatocytes during primary cu...
10434069 - Receding cytochrome p450 activity in disassembling hepatocyte spheroids.
9417869 - Aging reduces the numbers of hepatocytes synthesizing dna in response to egf and epinep...
16218189 - Long-term preservation of high endocytic activity in primary cultures of pig liver sinu...
3332659 - Foetal-to-adult transitions in fibroblast phenotype: their possible relevance to the pa...
9610389 - Characteristics of smooth muscle cell lipoprotein binding proteins (p105/p130) as t-cad...
591869 - Mesodermal expansion after arrest of the edge in the area vasculosa of the chick.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Toxicology and applied pharmacology     Volume:  176     ISSN:  0041-008X     ISO Abbreviation:  Toxicol. Appl. Pharmacol.     Publication Date:  2001 Nov 
Date Detail:
Created Date:  2001-11-20     Completed Date:  2001-12-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0416575     Medline TA:  Toxicol Appl Pharmacol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  169-80     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Affiliation:
Environment Canada, National Wildlife Research Centre, Hull, Quebec, K1A 0H3, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Biological Assay*
Birds / embryology,  genetics*
Cells, Cultured
Chick Embryo
Chickens
DDT / pharmacology
DNA / chemistry
Estrogen Antagonists / pharmacology
Estrogens, Non-Steroidal / pharmacology
Ethinyl Estradiol / analogs & derivatives*,  pharmacology
Female
Gene Expression / drug effects
Hepatocytes / chemistry
Liver / embryology
Male
Molecular Sequence Data
RNA, Messenger / analysis*,  biosynthesis
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction*
Songbirds / embryology,  genetics
Vitellogenins / genetics*
Chemical
Reg. No./Substance:
0/Estrogen Antagonists; 0/Estrogens, Non-Steroidal; 0/RNA, Messenger; 0/Vitellogenins; 34816-55-2/moxestrol; 50-29-3/DDT; 57-63-6/Ethinyl Estradiol; 789-02-6/o,p'-DDT; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Low concentrations of inorganic mercury inhibit Ras activation during T cell receptor-mediated signa...
Next Document:  Mechanism of inhibition of lysyl hydroxylase activity by the organophosphates malathion and malaoxon...