Document Detail

A reverse transcriptase PCR technique for the detection and viability assessment of Kluyveromyces marxianus in yoghurt.
MedLine Citation:
PMID:  16995526     Owner:  NLM     Status:  MEDLINE    
A fast and sensitive reverse transcriptase PCR (RT-PCR) method was developed for the detection of viable Kluyveromyces marxianus in yoghurt. Yeast-specific primers were used with the RT-PCR to evaluate the suitability of 18S rRNA as a target for the detection of viable yeasts in pure culture and yoghurt. The RT-PCR assay was able to detect down to 10(2) CFU ml(-1) in yoghurt samples contaminated with viable yeast cells. Application of the RT-PCR method to commercial yoghurt samples demonstrated the utility of this technique for detection of low concentrations of viable yeast cells in naturally contaminated dairy products. The 18S rRNA molecule is an appropriate target for cell viability assessment because of its limited persistence after cell death and the resultant high level of sensitivity of the assay.
María Belén Mayoral; Rosario Martin; Pablo E Hernández; Isabel González; Teresa García
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of food protection     Volume:  69     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2006-09-25     Completed Date:  2006-10-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2210-6     Citation Subset:  IM    
Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain.
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MeSH Terms
Colony Count, Microbial / methods
DNA, Fungal / analysis
Food Contamination / analysis*
Food Microbiology
Kluyveromyces / growth & development,  isolation & purification*
RNA, Ribosomal, 18S / analysis
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Yogurt / microbiology*
Reg. No./Substance:
0/DNA, Fungal; 0/RNA, Ribosomal, 18S

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