Document Detail

The retinoic acid metabolising gene, CYP26B1, patterns the cartilaginous cranial neural crest in zebrafish.
MedLine Citation:
PMID:  17616924     Owner:  NLM     Status:  MEDLINE    
We have investigated the function of the retinoic acid metabolising enzyme, CYP26B1, by administering an antisense morpholino oligonucleotide to zebrafish embryos. The result was an alteration in the morphology of the embryo in those regions which express the gene, namely an embryo with a smaller head, correspondingly smaller hindbrain rhombomeres and severely reduced numbers of vagal brachiomotor neurons. Most strikingly, these embryos had defective or absent jaw cartilages implying a role for this enzyme in patterning or migration of the neural crest cells which give rise to this tissue type. In order to determine whether this phenotype resembles that of excess retinoic acid or a deficiency of retinoic acid, we compared the jaw defects following retinoic acid treatment or DEAB treatment, the latter being an inhibitor of retinoic acid synthesis. The effects of the inhibitor rather than excess retinoic acid most closely phenocopied the jaw defects seen with the Cyp26B1 morpholino which suggests that, at least in the zebrafish embryo, the action of CYP26B1 in the neural crest may not be simply to catabolise all-trans-RA.
Susan Reijntjes; Adam Rodaway; Malcolm Maden
Related Documents :
20154154 - Induction of peroxisomal proliferator-activated receptor gamma and peroxisomal prolifer...
7825644 - Role of androgens in the developmental biology of the pilosebaceous unit.
22440424 - Prediction of fat quality in pig carcasses by near-infrared spectroscopy.
762444 - Effect of retinoids on the differentiation of chick embryo metatarsal skin explants.
17245744 - Novel cholic acid functionalized star oligo/poly(dl-lactide)s for biomedical applications.
3892004 - Improved antagonists of luteinizing hormone-releasing hormone modified in position 7.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of developmental biology     Volume:  51     ISSN:  0214-6282     ISO Abbreviation:  Int. J. Dev. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-07-09     Completed Date:  2007-10-05     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  8917470     Medline TA:  Int J Dev Biol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  351-60     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cartilage / embryology,  metabolism*
Cytochrome P-450 Enzyme System / genetics,  metabolism*
DNA, Antisense / genetics
Embryo, Nonmammalian / embryology,  metabolism
Gene Expression Regulation, Developmental
Homeodomain Proteins / metabolism
Motor Neurons / metabolism
Neural Crest / embryology*,  metabolism*
Rhombencephalon / embryology,  metabolism
Transcription Factors / metabolism
Tretinoin / administration & dosage,  metabolism*,  pharmacology
Zebrafish / embryology*,  metabolism*
p-Aminoazobenzene / administration & dosage,  analogs & derivatives,  pharmacology
Grant Support
G9900989//Medical Research Council
Reg. No./Substance:
0/DNA, Antisense; 0/Distal-less homeobox proteins; 0/Homeodomain Proteins; 0/Transcription Factors; 2481-94-9/C.I. Solvent Yellow 56; 5688UTC01R/Tretinoin; 57X2AH42T1/p-Aminoazobenzene; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.-/cytochrome P-450 CYP26B1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  The dynamic changes of plasma neuropeptide y and neurotensin and their role in regulating cerebral h...
Next Document:  An activating mutation in the PDGF receptor-beta causes abnormal morphology in the mouse placenta.