Document Detail


The retinoic acid metabolising gene, CYP26B1, patterns the cartilaginous cranial neural crest in zebrafish.
MedLine Citation:
PMID:  17616924     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have investigated the function of the retinoic acid metabolising enzyme, CYP26B1, by administering an antisense morpholino oligonucleotide to zebrafish embryos. The result was an alteration in the morphology of the embryo in those regions which express the gene, namely an embryo with a smaller head, correspondingly smaller hindbrain rhombomeres and severely reduced numbers of vagal brachiomotor neurons. Most strikingly, these embryos had defective or absent jaw cartilages implying a role for this enzyme in patterning or migration of the neural crest cells which give rise to this tissue type. In order to determine whether this phenotype resembles that of excess retinoic acid or a deficiency of retinoic acid, we compared the jaw defects following retinoic acid treatment or DEAB treatment, the latter being an inhibitor of retinoic acid synthesis. The effects of the inhibitor rather than excess retinoic acid most closely phenocopied the jaw defects seen with the Cyp26B1 morpholino which suggests that, at least in the zebrafish embryo, the action of CYP26B1 in the neural crest may not be simply to catabolise all-trans-RA.
Authors:
Susan Reijntjes; Adam Rodaway; Malcolm Maden
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The International journal of developmental biology     Volume:  51     ISSN:  0214-6282     ISO Abbreviation:  Int. J. Dev. Biol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-07-09     Completed Date:  2007-10-05     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  8917470     Medline TA:  Int J Dev Biol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  351-60     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cartilage / embryology,  metabolism*
Cytochrome P-450 Enzyme System / genetics,  metabolism*
DNA, Antisense / genetics
Embryo, Nonmammalian / embryology,  metabolism
Gene Expression Regulation, Developmental
Homeodomain Proteins / metabolism
Motor Neurons / metabolism
Neural Crest / embryology*,  metabolism*
Phenotype
Rhombencephalon / embryology,  metabolism
Transcription Factors / metabolism
Tretinoin / administration & dosage,  metabolism*,  pharmacology
Zebrafish / embryology*,  metabolism*
p-Aminoazobenzene / administration & dosage,  analogs & derivatives,  pharmacology
Grant Support
ID/Acronym/Agency:
G9900989//Medical Research Council
Chemical
Reg. No./Substance:
0/DNA, Antisense; 0/Distal-less homeobox proteins; 0/Homeodomain Proteins; 0/Transcription Factors; 2481-94-9/C.I. Solvent Yellow 56; 5688UTC01R/Tretinoin; 57X2AH42T1/p-Aminoazobenzene; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.14.-/cytochrome P-450 CYP26B1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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