| The retinoblastoma-susceptibility gene product becomes phosphorylated in multiple stages during cell cycle entry and progression. | |
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MedLine Citation:
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PMID: 1531876 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The retinoblastoma-susceptibility gene product (RB) undergoes cell cycle-dependent phosphorylation and dephosphorylation. We characterized RB phosphorylation after mitogenic stimulation of primary human T lymphocytes, initially arrested in the G0 state. RB is phosphorylated in at least three steps when T cells are driven into the cell cycle. The first event occurs during mid G1 phase, the second during S phase, and the third in G2/M. Tryptic phosphopeptide mapping indicates that the different phosphorylation events occur, at least in part, on different residues in RB. Given the known relationship of the RB phosphorylation state to function, it is possible that RB regulates growth at multiple points in the cell cycle. |
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Authors:
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J A DeCaprio; Y Furukawa; F Ajchenbaum; J D Griffin; D M Livingston |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 89 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1992 Mar |
Date Detail:
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Created Date: 1992-04-07 Completed Date: 1992-04-07 Revised Date: 2010-09-07 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1795-8 Citation Subset: IM |
Affiliation:
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Dana-Farber Cancer Institute, Boston, MA 02115. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Blotting, Western CDC2 Protein Kinase / metabolism Cell Cycle* Cells, Cultured Cyclins / metabolism Genes, Retinoblastoma* Humans Lymphocyte Activation Peptide Mapping Phosphorylation Retinoblastoma Protein / metabolism* T-Lymphocytes / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Cyclins; 0/Retinoblastoma Protein; EC 2.7.11.22/CDC2 Protein Kinase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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